Mouse anti-Human NRF1 Monoclonal Antibody | anti-NRF1 antibody

NRF1

Cat. Num. AAA215113

• Gene Names: NRF1; ALPHA-PAL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: NRF1, Antibody; NRF1; alpha pal; Alpha palindromic-binding protein; Alpha-pal; locus control region factor 1; NFE2 related factor 1; Nuclear respiratory factor 1 (NFR-1); anti-NRF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: NRF1/2609
• Specificity: The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 503

• Applicable Applications for anti-NRF1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Positive Control: Ubiquitous expression, strongest in skeletal muscle.
• Immunogen: Recombinant full-length human NRF1 protein
• Cellular Localization: Nucleus.
• Hu-chromosome Location: 17q21.32
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human prostate carcinoma stained with NRF1 Mouse Monoclonal Antibody (NRF1/2609).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human colon carcinoma stained with NRF1 Mouse Monoclonal Antibody (NRF1/2609).)

WB (Western Blot)

(Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using NRF1 Mouse Monoclonal Antibody (NRF1/2609).)

SDS-PAGE

(SDS-PAGE Analysis Purified NRF1 Mouse Monoclonal Antibody (NRF1/2609). Confirmation of Purity and Integrity of Antibody.)

References

Sakamoto, H, et al. Clinical Cancer Research U.S. National Library of Medicine, Aug. 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 93141039
• NCBI GeneID: 4899
• NCBI Accession #: NP_001035199.1
• NCBI GenBank Nucleotide #: NP_001035199.1
• UniProt Accession #: Q16656
• Molecular Weight: 30kDa (bZIP region); 65-120kDa (glycosylated)
• NCBI Official Full Name: nuclear respiratory factor 1 isoform 1
• NCBI Official Synonym Full Names: nuclear respiratory factor 1
• NCBI Official Symbol: NRF1
• NCBI Official Synonym Symbols: ALPHA-PAL
• NCBI Protein Information: nuclear respiratory factor 1
• UniProt Protein Name: Nuclear respiratory factor 1
• UniProt Gene Name: NRF1
• UniProt Synonym Gene Names: NRF-1; Alpha-pal
• UniProt Entry Name: NRF1_HUMAN