Rabbit anti-Human IGFBP3 Polyclonal Antibody | anti-IGFBP3 antibody

Anti-IGFBP3 antibody

Cat. Num. AAA175212

• Gene Names: IGFBP3; IBP3; BP-53
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: IGFBP3; Polyclonal Antibody; Anti-IGFBP3 antibody; Insulin-like growth factor-binding protein 3(IBP-3/IGF-binding protein 3/IGFBP-3); insulin-like growth factor binding protein 3; Acid stable subunit of the 140 K IGF complex antibody; Binding protein 29 antibody; Binding protein 53 antibody; BP 53 antibody; BP53 antibody; Growth hormone dependent binding protein antibody; IBP 3 antibody; IBP-3 antibody; IBP3 antibody; IBP3_HUMAN antibody; IGF binding protein 3 antibody; IGF-binding protein 3 antibody; IGFBP 3 antibody; IGFBP-3 antibody; IGFBP3 antibody; Insulin Like Growth Factor Binding Protein 3 antibody; Insulin-like growth factor-binding protein 3 antibody; anti-IGFBP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 291

• Applicable Applications for anti-IGFBP3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human IGFBP3 (182-198aa IKKGHAKDSQRYKVDYE).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-IGFBP-3 antibody, MBS175212, Western blottingAll lanes: Anti IGFBP-3 (MBS175212) at 0.5ug/mlLane 1: 293T Whole Cell Lysate at 40ugLane 2: MCF- 7 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: SW620 Whole Cell Lysate at 40ugPredicted bind size: 32KDObserved bind size: 40KD )

Immunohistochemistry (IHC)

(Anti-IGFBP-3 antibody, MBS175212, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-IGFBP3 antibody

Description: Rabbit IgG polyclonal antibody for Insulin-like growth factor-binding protein 3(IGFBP3) detection. Tested with WB, IHC-P in Human.
Background: IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein(IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit(IGFALS) and either insulin-like growth factor(IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

NCBI and Uniprot Product Information

• NCBI GI #: 146327827
• NCBI GeneID: 3486
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• Molecular Weight: 32,223 Da
• NCBI Official Full Name: Insulin-like growth factor-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; fibronectin binding; metal ion binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of catalytic activity; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; apoptosis; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; negative regulation of signal transduction; osteoblast differentiation; negative regulation of cell proliferation; cellular protein metabolic process; positive regulation of MAPKKK cascade; negative regulation of protein amino acid phosphorylation; regulation of cell growth