Rabbit anti-Mouse RB1CC1 Polyclonal Antibody | anti-RB1CC1 antibody

RB1CC1, ID (RB1CC1, KIAA0203, RBICC, RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200kD) (PE)

Cat. Num. AAA6340596

• Gene Names: RB1CC1; CC1; FIP200
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RB1CC1; Polyclonal Antibody; ID (RB1CC1; KIAA0203; RBICC; RB1-inducible coiled-coil protein 1; FAK family kinase-interacting protein of 200kD) (PE); FAK family kinase-interacting protein of 200kD; anti-RB1CC1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RB1CC1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RB1CC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-523 amino acids from the Central region of human RB1CC1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RB1CC1 Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the RB1CC1 antibody detected the RB1CC1 protein (arrow).)

Related Product Information for anti-RB1CC1 antibody

The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-RB1CC1 antibody

Antibodies; Protein Kinases

References

Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Chano, T., et al. PLoS ONE 5 (6), E11404 (2010): Paun, B.C., et al. PLoS ONE 4 (11), E7715 (2009): Chan, E.Y. Sci Signal 2 (84), PE51 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 134304846
• NCBI GeneID: 9821
• NCBI Accession #: NP_001077086.1; NP_055596.3
• NCBI GenBank Nucleotide #: NM_001083617.1
• UniProt Accession #: Q8TDY2; Q86YR4; Q8WVU9; Q92601
• Molecular Weight: 183,091 Da
• NCBI Official Full Name: RB1-inducible coiled-coil protein 1 isoform 2
• NCBI Official Synonym Full Names: RB1-inducible coiled-coil 1
• NCBI Official Symbol: RB1CC1
• NCBI Official Synonym Symbols: CC1; FIP200
• NCBI Protein Information: RB1-inducible coiled-coil protein 1; 200 kDa FAK family kinase-interacting protein; FAK family kinase-interacting protein of 200 kDa
• UniProt Protein Name: RB1-inducible coiled-coil protein 1
• Protein Family: RB1-inducible coiled-coil protein
• UniProt Gene Name: RB1CC1
• UniProt Synonym Gene Names: KIAA0203; RBICC; FIP200
• UniProt Entry Name: RBCC1_HUMAN

NCBI Description

The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009]

Uniprot Description

RB1CC1: a putative transcription factor that functions as a key regulator of Rb expression. Required for autophagosome formation. Contains a nuclear localization signal, a leucine zipper motif and a coiled-coil structure. Thought to play a biological role in controlling cell growth and progression of various cancers. Probably involved in the tumorigenesis of breast cancer. It is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. Plays a crucial role in muscular differentiation and required for myogenic differentiation. Part of a complex consisting of ATG13, ULK1 and RB1CC1. This complex associates with ATG101. Under starvation conditions, is localized to phagophores. Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay.

Protein type: DNA-binding; Autophagy; Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nuclear membrane; cytoplasm; pre-autophagosomal structure membrane; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell size; heart development; JNK cascade; regulation of autophagy; positive regulation of protein amino acid phosphorylation; liver development; cell cycle; autophagic vacuole formation

Disease: Breast Cancer