Human IGFBP-3 ELISA Kit | IGFBP-3 elisa kit

IGFBP-3 Human ELISA Kit

• Gene Names: IGFBP3; IBP3; BP-53
• Reactivity: Human
• Applications: ELISA
• Synonyms: IGFBP-3; IGFBP-3 Human ELISA Kit; Human IGFBP-3; IGFBP-3 elisa kit

• Reactivity: Human

• Applicable Applications for IGFBP-3 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of IGFBP-3 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP-3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGFBP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-3 can be calculated.

Background: Insulin-like growth factor-binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor-binding protein (IGFBP) family. This protein has an IGFBP domain and a thyroglobulin type-I domain. It forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. It plays a role in controlling angiogenesis and cell responses in the human corpus luteum by autocrine/paracrine mechanisms. IGFBP3 protein levels decrease during the progression of prostate cancer from benign prostatic hyperplasia (BPH) to its metastatic form.

NCBI and Uniprot Product Information

• NCBI GI #: 62243248
• NCBI GeneID: 3486
• NCBI Accession #: NP_001013416.1
• NCBI GenBank Nucleotide #: NM_001013398.1
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• Molecular Weight: 31,674 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 3 isoform a
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Ref.16

Subunit structure: Interacts with XLKD1

By similarity. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219. Ref.10 Ref.12 Ref.16

Subcellular location: Secreted Ref.16.

Tissue specificity: Expressed by most tissues. Present in plasma.

Developmental stage: IGFBP3 levels are higher during extrauterine life and peak during puberty.

Induction: Up-regulated in the presence of IGF1, insulin and other growth-stimulating factors such as growth hormone, EGF and phorbol esters.

Domain: The thyroglobulin type-1 domain mediates interaction with HN.

Post-translational modification: Phosphorylation sites are present in the extracellular medium.

Sequence similarities: Contains 1 IGFBP N-terminal domain.Contains 1 thyroglobulin type-1 domain.