Western Blot (WB)
(Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149). Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).)
Rabbit Phosphoserine Polyclonal Antibody | anti-PSPH antibody
Phosphoserine Antibody: HRP
Gene Names
PSPH; PSP; PSPHD
Reactivity
Species Independent
Applications
Western Blot, ELISA
Synonyms
Phosphoserine; Polyclonal Antibody; Phosphoserine Antibody: HRP; anti-PSPH antibody
Host
Rabbit
Reactivity
Species Independent
Clonality
Polyclonal
Form/Format
Affinity Purified, HRP conjugated
Concentration
250ug/mL (varies by lot)
Sequence Length
225
Applicable Applications for anti-PSPH antibody
Western Blot (WB), ELISA (EIA)
Application Notes
1:500 (WB)
Certificate of Analysis
2ug/mL of SPC-151 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1uM okadaic acid.
Conjugate
HRP
Storage Buffer
HRP Stabilizing buffer: The HRP stabilizing buffer contains PBS, 0.1% BSA, 0.01% proclin 150, and 50% glycerol
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149). Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).)
Western Blot (WB)
(Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149). Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).)
Related Product Information for anti-PSPH antibody
Background Info: Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Scientific Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Scientific Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Product Categories/Family for anti-PSPH antibody
References
1. Goto H. et al. (2005). Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001). Nature 411: 355- 365. 3. Downward J. (2001). Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999). Cell 97: 675-678. 5. Ostrovsky PC. (1995). Genes Dev. 9(16): 2034-2041.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,008 Da
NCBI Official Full Name
phosphoserine phosphatase
NCBI Official Synonym Full Names
phosphoserine phosphatase
NCBI Official Symbol
PSPH
NCBI Official Synonym Symbols
PSP; PSPHD
NCBI Protein Information
phosphoserine phosphatase; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase
UniProt Protein Name
Phosphoserine phosphatase
Protein Family
UniProt Gene Name
PSPH
UniProt Synonym Gene Names
PSP; PSPase
UniProt Entry Name
SERB_HUMAN
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Product Notes
The PSPH psph (Catalog #AAA805180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phosphoserine Antibody: HRP reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoserine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). 1:500 (WB). Researchers should empirically determine the suitability of the PSPH psph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphoserine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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