Rabbit IGFBP-9 Polyclonal Antibody | anti-IGFBP-9 antibody

RABBIT ANTI HUMAN IGFBP-9

Cat. Num. AAA222933

• Gene Names: NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: IGFBP-9; Polyclonal Antibody; RABBIT ANTI HUMAN IGFBP-9; anti-IGFBP-9 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 357

• Applicable Applications for anti-IGFBP-9 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Immunogen: Recombinant human IGFBP-9; Buffer Solution
• Antiserum Preparation: Antiserum to human IGFBP-9 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human breast invasive carcinoma stained with Rabbit anti Human IGFBP-9 (MBS222933) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Testing Data #2

(Formalin fixed, paraffin embedded human breast invasive carcinoma stained with Rabbit anti Human IGFBP-9 (MBS222933) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-IGFBP-9 antibody

Rabbit anti Human IGFBP-9 antibody recognizes human IGFBP-9 (insulin-like growth factor binding protein- 9), also known as NOV/CCN3, a 326 amino acid cysteine-rich secreted regulatory protein and member of the CCN family, containing the domains IGFBP, VWFC, C-Terminal cysteine knot-like and thrombospondin type I repeat. IGFBP-9 is an inducer of cell migration and adhesion and shows increased expression in Wilm's tumours and nephroblastomas. In contrast, decreased expression of IGFBP-9 correlates with an increase in tumourgenicity in certain cancers including childhood adrenocortical tumours (ACT).

NCBI and Uniprot Product Information

• NCBI GI #: 4505423
• NCBI GeneID: 4856
• NCBI Accession #: NP_002505.1
• NCBI GenBank Nucleotide #: NM_002514.3
• UniProt Accession #: P48745; Q6I9S3; Q96BY5; Q9UDE4; B2R5X7
• Molecular Weight: 39,162 Da
• NCBI Official Full Name: protein NOV homolog
• NCBI Official Synonym Full Names: nephroblastoma overexpressed
• NCBI Official Symbol: NOV
• NCBI Official Synonym Symbols: CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• NCBI Protein Information: protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
• UniProt Protein Name: Protein NOV homolog
• UniProt Gene Name: NOV
• UniProt Synonym Gene Names: CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
• UniProt Entry Name: NOV_HUMAN

NCBI Description

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]

Uniprot Description

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction