Typical Testing Data/Standard Curve (for reference only)
(Human NOV/CCN3 PicoKine ELISA Kit standard curve )
Human NOV/CCN3 ELISA Kit | NOV elisa kit
Human NOV/CCN3 PicoKine ELISA Kit
Gene Names
NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
Reactivity
Human
Synonyms
NOV/CCN3; Human NOV/CCN3 PicoKine ELISA Kit; Protein NOV homolog; nephroblastoma overexpressed; CCN family member 3; CCN3; IBP-9; IGF-binding protein 9; IGFBP-9; IGFBP9; Insulin-like growth factor-binding protein 9; Nephroblastoma overexpressed; Nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NOV; NOV_HUMAN; NovH; NOV elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human NOV/CCN3
Sequence Length
357
Samples
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human NOV/CCN3 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human NOV/CCN3 PicoKine ELISA Kit standard curve )
Related Product Information for NOV elisa kit
Principle of the Assay: Human NOV/CCN3 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for NOV/CCN3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOV/CCN3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NOV/CCN3 amount of sample captured in plate.
Background/Introduction: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the identification of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
Background/Introduction: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the identification of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,162 Da
NCBI Official Full Name
Protein NOV homolog
NCBI Official Synonym Full Names
nephroblastoma overexpressed
NCBI Official Symbol
NOV
NCBI Official Synonym Symbols
CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
NCBI Protein Information
protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
UniProt Protein Name
Protein NOV homolog
Protein Family
UniProt Gene Name
NOV
UniProt Synonym Gene Names
CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
UniProt Entry Name
NOV_HUMAN
NCBI Description
The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]
Uniprot Description
NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8q24.1
Cellular Component: proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite
Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity
Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction
Research Articles on NOV
Similar Products
Product Notes
The Human NOV nov (Catalog #AAA177346) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177346 ELISA Kit recognizes Human NOV. It is sometimes possible for the material contained within the vial of "NOV/CCN3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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