NOV/CCN3 Active Protein | NOV active protein

NOV/CCN3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120146

• Gene Names: NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• Purity: >94% as determined by SDS-PAGE
• Synonyms: NOV/CCN3; NOV/CCN3 Protein; Human; Recombinant (His Tag); Human CCN3/NOV Protein (His Tag); CCN3 Protein; IBP-9 Protein; IGFBP-9 Protein; IGFBP9 Protein; NOVh Protein; nephroblastoma overexpressed; NOV active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10mM Imidazole, 10% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the ha
• Sequence: Met1-Met357

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cells. When cells are added to CCN3-coated plates (10ug/ml, 100uL/well), >50% cells will adhere specifically after 60 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of human NOV (NP_002505.1)(Met 1-Met 357) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for NOV active protein

Background: Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment.

References

Manara MC, et al. (2002) The expression of ccn3(nov) gene in musculoskeletal tumors. Am J Pathol. 160(3): 849-59.Lin CG, et al. (2003) CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family. J Biol Chem. 278(26): 24200-8.Vallacchi V, et al. (2009) CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. Cancer Res. 68(3): 715-23.Sin WC, et al. (2009) Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization. J Biol Chem. 284(43): 29935-44.McCallum L, et al. (2009) CCN3--a key regulator of the hematopoietic compartment. Blood Rev. 23(2): 79-85.

NCBI and Uniprot Product Information

• NCBI GI #: 4505423
• NCBI GeneID: 4856
• NCBI Accession #: NP_002505.1
• NCBI GenBank Nucleotide #: NM_002514.3
• UniProt Accession #: P48745; Q6I9S3; Q96BY5; Q9UDE4; B2R5X7
• Molecular Weight: 39,162 Da
• NCBI Official Full Name: protein NOV homolog
• NCBI Official Synonym Full Names: nephroblastoma overexpressed
• NCBI Official Symbol: NOV
• NCBI Official Synonym Symbols: CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• NCBI Protein Information: protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
• UniProt Protein Name: Protein NOV homolog
• Protein Family: Protein
• UniProt Gene Name: NOV
• UniProt Synonym Gene Names: CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
• UniProt Entry Name: NOV_HUMAN

NCBI Description

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]

Uniprot Description

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: proteinaceous extracellular matrix; intracellular membrane-bound organelle; cell soma; axon; dendrite

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; cell adhesion; signal transduction