Rabbit anti-Human SLC22A6 / OAT1 Polyclonal Antibody | anti-SLC22A6 / OAT1 antibody

Anti-SLC22A6 / OAT1 Antibody (aa520-549) IHC-plus

Cat. Num. AAA2400268

• Gene Names: SLC22A6; OAT1; PAHT; HOAT1; ROAT1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A Purified
• Synonyms: SLC22A6 / OAT1; Polyclonal Antibody; Anti-SLC22A6 / OAT1 Antibody (aa520-549) IHC-plus; Rabbit Polyclonal to Human SLC22A6 / OAT1; Human SLC22A6 / OAT1; HOAT1; HPAHT; Organic anion transporter 1; P-aminohippurate transporter; PAHT; PAH transporter; ROAT1; HROAT1; anti-SLC22A6 / OAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This SLC22A6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the C-terminal region of human SLC22A6.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 563

• Applicable Applications for anti-SLC22A6 / OAT1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:10 - 1:50); IHC-P (1:50); WB (1:1000)
• Immunogen Species: SLC22A6 / OAT1 antibody was raised against Human.
• Epitope: aa513-541
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-SLC22A6 / OAT1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

(Western blot of SLC22A6 Antibody in CEM cell line lysates (35 ug/lane). SLC22A6 (arrow) was detected using the purified antibody.)

Flow Cytometry (FC/FACS)

(SLC22A6 Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

NCBI and Uniprot Product Information

• NCBI GI #: 20070188
• NCBI GeneID: 9356
• NCBI Accession #: NP_004781.2
• NCBI GenBank Nucleotide #: NM_004790.4
• UniProt Accession #: Q4U2R8; O95187; O95742; Q7LDA0; Q8N192; Q9NQA6; Q9NQC2; Q9UBG6; Q9UEQ8; A8MY93; B2D0R6
• NCBI Official Full Name: solute carrier family 22 member 6 isoform a
• NCBI Official Synonym Full Names: solute carrier family 22 (organic anion transporter), member 6
• NCBI Official Symbol: SLC22A6
• NCBI Official Synonym Symbols: OAT1; PAHT; HOAT1; ROAT1
• NCBI Protein Information: solute carrier family 22 member 6
• UniProt Protein Name: Solute carrier family 22 member 6
• UniProt Gene Name: SLC22A6
• UniProt Synonym Gene Names: OAT1; PAHT; hOAT1; hPAHT; hROAT1
• UniProt Entry Name: S22A6_HUMAN

NCBI Description

The protein encoded by this gene is involved in the sodium-dependent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and may be localized to the basolateral membrane. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC22A6: Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS). Mediates the sodium-independent uptake of p- aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido- 3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro- phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2- furanpropionate (CMPF), cidofovir, adefovir, 9-(2- phosphonylmethoxyethyl) guanine (PMEG), 9-(2- phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p- chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid. PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: basolateral plasma membrane; caveola; integral to plasma membrane; plasma membrane; protein complex

Molecular Function: chloride ion binding; inorganic anion exchanger activity; organic anion transmembrane transporter activity; protein binding; protein homodimerization activity; sodium-independent organic anion transmembrane transporter activity

Biological Process: alpha-ketoglutarate transport; organic anion transport; protein homooligomerization; response to methotrexate; sodium-independent organic anion transport; transmembrane transport