Rabbit anti-Human IGF-II Polyclonal Antibody | anti-IGF-II antibody

IGF-II Antibody

Cat. Num. AAA840925

• Gene Names: IGF2; GRDF; IGF-II; PP9974; C11orf43
• Reactivity: Human
• Applications: Western Blot, Neutralization Assay
• Purity: Affinity purified
• Synonyms: IGF-II; Polyclonal Antibody; IGF-II Antibody; IGF2; IGF-2; IGF 2; Insulin like growth factor-2; Insulin like growth factor 2; anti-IGF-II antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) antigen affinity purified rabbit anti-human IGF-II polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 236

• Applicable Applications for anti-IGF-II antibody: Western Blot (WB), Neutralization
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Recombinant human IGF-II can be used as a positive control. Other applications have not been determined.
• Immunogen: E. coli expressed human IGF-II
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-IGF-II antibody

Background: IGF-II (Insulin-like Growth Factor-II) is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types. Human IGF-II is a 7.5 kDa protein containing 67 amino acid residues.

NCBI and Uniprot Product Information

• NCBI GI #: 189083846
• NCBI GeneID: 3481
• NCBI Accession #: NP_001121070
• NCBI GenBank Nucleotide #: NM_001127598.2
• Molecular Weight: 26,331 Da
• NCBI Official Full Name: insulin-like growth factor II isoform 2
• NCBI Official Synonym Full Names: insulin-like growth factor 2
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: GRDF; IGF-II; PP9974; C11orf43
• NCBI Protein Information: insulin-like growth factor II
• UniProt Protein Name: Insulin-like growth factor II
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: IGF-II
• UniProt Entry Name: IGF2_HUMAN

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; insulin receptor binding; receptor activator activity

Biological Process: positive regulation of catalytic activity; response to nicotine; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; skeletal development; response to drug; response to nutrient levels; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; response to ethanol; cellular protein metabolic process; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; blood coagulation

Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1