Human Reactive oxygen species modulator 1 ELISA Kit | ROMO1 elisa kit

Human Reactive oxygen species modulator 1 ELISA Kit

Cat. Num. AAA764414

• Gene Names: ROMO1; MTGM; MTGMP; C20orf52; bA353C18.2
• Reactivity: Human
• Synonyms: Reactive oxygen species modulator 1; Human Reactive oxygen species modulator 1 ELISA Kit; ROMO1/Reactive oxygen species modulator 1/ROS modulator 1/Protein MGR2 homolog/Mitochondrial targeting GxxxG motif protein/MTGM/Protein MGR2 homolog/Glyrichin; ROMO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ROMO1 . No significant cross-reactivity or interference between ROMO1 and analogues was observed.
• Sequence Length: 79

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 31.2-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ROMO1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ROMO1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ROMO1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ROMO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ROMO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ROMO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ROMO1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 18152785
• NCBI GeneID: 140823
• NCBI Accession #: NP_542786.1
• NCBI GenBank Nucleotide #: NM_080748.2
• Molecular Weight: 5,900 Da
• NCBI Official Full Name: reactive oxygen species modulator 1
• NCBI Official Synonym Full Names: reactive oxygen species modulator 1
• NCBI Official Symbol: ROMO1
• NCBI Official Synonym Symbols: MTGM; MTGMP; C20orf52; bA353C18.2
• NCBI Protein Information: reactive oxygen species modulator 1
• UniProt Protein Name: Reactive oxygen species modulator 1
• Protein Family: Reactive oxygen species modulator
• UniProt Gene Name: ROMO1
• UniProt Synonym Gene Names: C20orf52; ROS modulator 1; MTGM
• UniProt Entry Name: ROMO1_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial membrane protein that is responsible for increasing the level of reactive oxygen species (ROS) in cells. The protein also has antimicrobial activity against a variety of bacteria by inducing bacterial membrane breakage. [provided by RefSeq, Nov 2014]

Uniprot Description

ROMO1: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Belongs to the MGR2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Mitochondrial; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Biological Process: defense response to bacterium; positive regulation of cell proliferation; replicative cell aging