Human Insulin-like growth factor II ELISA Kit | IGF-2 elisa kit

Human Insulin-like growth factor II ELISA Kit

Cat. Num. AAA760585

• Gene Names: IGF2; GRDF; IGF-II; PP9974; C11orf43
• Reactivity: Human
• Synonyms: Insulin-like growth factor II; Human Insulin-like growth factor II ELISA Kit; IGF-2/IGF2/IGF-II; IGF-2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IGF-2. No significant cross-reactivity or interference between IGF-2 and analogues was observed.
• Sequence Length: 180

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/ml
• Sensitivity: <37.5pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF- 2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF- 2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for IGF-2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IGF-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IGF-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGF-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGF-2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 914192
• NCBI GeneID: 3481
• Molecular Weight: 26,331 Da
• NCBI Official Full Name: insulin-like growth factor II
• NCBI Official Synonym Full Names: insulin like growth factor 2
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: GRDF; IGF-II; PP9974; C11orf43
• NCBI Protein Information: insulin-like growth factor II
• UniProt Protein Name: Insulin-like growth factor II
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: IGF-II
• UniProt Entry Name: IGF2_HUMAN

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding; protein serine/threonine kinase activator activity; receptor activator activity

Biological Process: blood coagulation; cellular protein metabolic process; exocrine pancreas development; female pregnancy; genetic imprinting; glucose metabolic process; insulin receptor signaling pathway; memory; multicellular organismal development; negative regulation of natural killer cell mediated cytotoxicity; organ morphogenesis; ossification; platelet activation; platelet degranulation; positive regulation of activated T cell proliferation; positive regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of glycogen biosynthetic process; positive regulation of insulin receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of multicellular organism growth; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to drug; response to estradiol stimulus; response to ethanol; response to nicotine; response to nutrient levels; response to radiation; skeletal development; striated muscle cell differentiation

Disease: Beckwith-wiedemann Syndrome; Growth Restriction, Severe, With Distinctive Facies; Silver-russell Syndrome; Wilms Tumor 1