Rabbit anti-Human IFT20 Polyclonal Antibody | anti-IFT20 antibody

Anti-IFT20 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103965

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: IFT20; Polyclonal Antibody; Anti-IFT20 Antibody; Rabbit Polyclonal; IFT20/intraflagellar transport 20 Antibody; Rabbit PAb; Antigen Affinity Purified; intraflagellar transport 20; anti-IFT20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human IFT20/intraflagellar transport 20
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-IFT20 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human IFT20/intraflagellar transport 20
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human IFT20/intraflagellar transport 20, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human IFT20 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human IFT20 in human adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-IFT20 antibody

Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles motor along ciliary microtubules. IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. IFT20 is the smallest member of the intraflagellar transport protein (IFT) complex B. It is involved in cilia formation. IFT20 in the early stage of T-cell development inhibited collagen-induced arthritis (CIA) development through regulating T-cell development and the expression of critical cytokines. The intraflagellar transport protein 20 (IFT20), a component of the intraflagellar transport system, controls polarized traffic during immune synapse assembly. IFT20 is therefore required for the delivery of the intracellular pool of LAT to the immune synapse in naive primary T lymphocytes and for effective T-cell responses in vivo. IFT20 is essential for male fertility and spermiogenesis in mice, and its major function is to transport cargo proteins for sperm flagella formation.

NCBI and Uniprot Product Information

• NCBI GI #: 392050749
• NCBI GeneID: 90410
• NCBI Accession #: NP_001254703.1
• NCBI GenBank Nucleotide #: NM_001267774.1
• UniProt Accession #: Q8IY31; Q5GLZ2; Q9BUG5; J3QL09
• Molecular Weight: 12,969 Da
• NCBI Official Full Name: intraflagellar transport protein 20 homolog isoform 1
• NCBI Official Synonym Full Names: intraflagellar transport 20
• NCBI Official Symbol: IFT20
• NCBI Protein Information: intraflagellar transport protein 20 homolog; intraflagellar transport 20 homolog; intraflagellar transport protein IFT20
• UniProt Protein Name: Intraflagellar transport protein 20 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT20
• UniProt Synonym Gene Names: hIFT20
• UniProt Entry Name: IFT20_HUMAN

NCBI Description

This gene encodes a intraflagellar transport protein important for intracellular transport. The encoded protein forms part of a complex involved in trafficking of proteins from the Golgi body, including recycling of immune signalling components (Finetti et al., PubMed: 19855387). This gene is part of a complex set of sense-antisense loci that may be co-regulated. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 14.[provided by RefSeq, Jun 2012]

Uniprot Description

IFT20: Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: centriole; microtubule cytoskeleton; Golgi membrane; Golgi apparatus; centrosome; photoreceptor outer segment; photoreceptor connecting cilium; cilium

Molecular Function: protein binding; opsin binding

Biological Process: smoothened signaling pathway; centrosome localization; organelle organization and biogenesis; protein localization in Golgi apparatus; intraflagellar transport; cilium biogenesis; visual learning; kidney development; cardiac muscle cell differentiation