Typical Testing Data/Standard Curve (for reference only)
Porcine Chemokine C-C motif ligand 8 ELISA Kit | CCL-8 elisa kit
Porcine Chemokine C-C motif ligand 8 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CCL8 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CCL8 antibody and an CCL8-HRP conjugate. The assay sample and buffer are incubated together with CCL8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL8 concentration since CCL8 from samples and CCL8-HRP conjugate compete for the anti-CCL8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL8 from the sample, fewer sites are left to bind CCL8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL8 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]
Uniprot Description
CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: extracellular space
Molecular Function: heparin binding; chemokine activity; protein kinase activity; phospholipase activator activity
Biological Process: positive regulation of catalytic activity; cellular calcium ion homeostasis; exocytosis; cell-cell signaling; calcium ion transport; response to virus; immune response; inflammatory response; signal transduction; chemotaxis; protein amino acid phosphorylation
Research Articles on CCL-8
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Product Notes
The Porcine CCL-8 ccl8 (Catalog #AAA746289) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746289 ELISA Kit recognizes Porcine CCL-8. It is sometimes possible for the material contained within the vial of "Chemokine C-C motif ligand 8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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