Goat CAV1 Polyclonal Antibody | anti-CAV1 antibody

Caveolin-1 Polyclonal Antibody

Cat. Num. AAA448024

• Gene Names: CAV1; CGL3; PPH3; BSCL3; VIP21; MSTP085
• Reactivity: Reacts against human, canine and mouse proteins
• Applications: Immunofluorescence, Western Blot
• Synonyms: CAV1; Polyclonal Antibody; Caveolin-1 Polyclonal Antibody; Anti-CAV1; caveolin; caveolae protein 1; cell growth-inhibiting protein 32 antibody; anti-CAV1 antibody

• Host: Goat
• Reactivity: Reacts against human, canine and mouse proteins
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a band of approximately 25 kDa by Western blot in the following cell lysate: hCat, MDCK and 3T3.
• Form/Format: Polyclonal antibody supplied as (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Sequence Length: 147

• Applicable Applications for anti-CAV1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: 1:500-1:2,000; Immunofluorescence: 1:25-1:250; IHC (Paraffin): 1:200-1:1000; IHC (frozen): 1:200-1:1000
• Gene Identifier: ENSG00000105974
• Immunogen: Recombinant peptide derived from within residues 100 aa to the N-terminus of human CAV1 produced in E. coli.
• Antigen: Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human CAV1 produced in E. coli.
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Store at -20 degree C for long term storage. Store at 2-8 degree C for up to one month.

Reactivity Chart

Testing Data

Related Product Information for anti-CAV1 antibody

Caveolin-1

Goat polyclonal to Caveolin-1 - caveolae marker. This scaffolding protein is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression.

NCBI and Uniprot Product Information

• NCBI GI #: 290542363
• NCBI GeneID: 857
• NCBI Accession #: NP_001166368.1
• NCBI GenBank Nucleotide #: NM_001172897.1
• UniProt Accession #: Q03135; Q9UGP1; Q9UNG1; Q9UQH6
• Molecular Weight: 20,472 Da
• NCBI Official Full Name: caveolin-1 isoform beta
• NCBI Official Synonym Full Names: caveolin 1, caveolae protein, 22kDa
• NCBI Official Symbol: CAV1
• NCBI Official Synonym Symbols: CGL3; PPH3; BSCL3; VIP21; MSTP085
• NCBI Protein Information: caveolin-1; cell growth-inhibiting protein 32
• UniProt Protein Name: Caveolin-1
• Protein Family: Caveolin
• UniProt Gene Name: CAV1
• UniProt Synonym Gene Names: CAV
• UniProt Entry Name: CAV1_HUMAN

NCBI Description

The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]

Uniprot Description

Function: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity

By similarity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Ref.8 Ref.13

Subunit structure: Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and STX1A. Interacts with rotavirus A NSP4. Interacts (via the N-terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with PACSIN2

By similarity. Interacts with BMX and BTK. Ref.8 Ref.9 Ref.11 Ref.12 Ref.13

Subcellular location: Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane › caveola; Peripheral membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Ref.6 Ref.8 Ref.13

Tissue specificity: Expressed in muscle and lung, less so in liver, brain and kidney.

Post-translational modification: The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Ref.6Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. Ref.6 Ref.10

Involvement in disease: Congenital generalized lipodystrophy 3 (CGL3) [MIM:612526]: An autosomal recessive disorder characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.14Pulmonary hypertension, primary, 3 (PPH3) [MIM:615343]: A rare disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial pulmonary hypertension is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.22

Sequence similarities: Belongs to the caveolin family.