Rabbit anti-Human HUB1 Polyclonal Antibody | anti-HUB1 antibody

HUB1, CT (UBL5) (PE)

Cat. Num. AAA6308913

• Gene Names: UBL5; HUB1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HUB1; Polyclonal Antibody; CT (UBL5) (PE); UBL5; anti-HUB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HUB1
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HUB1 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HUB1(UBL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HUB1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

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Related Product Information for anti-HUB1 antibody

UBL5 is a widely expressed human protein that is strongly conserved across phylogeny. Orthologs of UBL5 occur in every eukaryotic genome characterized to date. The yeast ortholog of UBL5, HUB1, was reported to be a ubiquitin-like protein modifier important for modulation of protein function. However, unlike ubiquitin and all other ubiquitin-like modifiers, UBL5 and its yeast ortholog HUB1 both contain a C-terminal di-tyrosine motif followed by a single variable residue instead of the characteristic di-glycine found in all other ubiquitin-like modifiers. Here we describe the three-dimensional structure of UBL5 determined by NMR. The overall structure of the protein was found to be very similar to ubiquitin despite the low approximately 25% residue similarity. The signature C-terminal di-tyrosine residues in UBL5 are involved in the final beta sheet of the protein. This is very different to the di-glycine motif found in ubiquitin, which extends beyond the final beta sheet. In addition, we have confirmed an earlier report of an interaction between UBL5 and the cyclin-like kinase, CLK4, which we have determined is specific and does not extend to other cyclin-like kinase family members.

Product Categories/Family for anti-HUB1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13236510
• NCBI GeneID: 59286
• NCBI Accession #: NP_077268
• NCBI GenBank Nucleotide #: NM_024292.3
• UniProt Accession #: Q9BZL1; Q2NL89
• Molecular Weight: 10.7 kDa (93aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin-like protein 5
• NCBI Official Synonym Full Names: ubiquitin like 5
• NCBI Official Symbol: UBL5
• NCBI Official Synonym Symbols: HUB1
• NCBI Protein Information: ubiquitin-like protein 5
• UniProt Protein Name: Ubiquitin-like protein 5
• Protein Family: Ubiquitin-like modifier
• UniProt Gene Name: UBL5
• UniProt Entry Name: UBL5_HUMAN

NCBI Description

This gene encodes a member of a group of proteins similar to ubiquitin. The encoded protein is not thought to degrade proteins like ubiquitin but to affect their function through being bound to target proteins by an isopeptide bond. The gene product has been studied as a link to predisposition to obesity based on its expression in Psammomys obesus, the fat sand rat, which is an animal model for obesity studies. Variation in this gene was found to be significantly associated with some metabolic traits (PMID: 15331561) but not associated with childhood obesity (PMID: 19189687). Pseudogenes of this gene are located on chromosomes 3, 5 and 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2013]

Uniprot Description

UBL5: a widely expressed and highly conserved protein. Orthologs of UBL5 occur in all characterized eukaryotic genomes. Specifically interacts with CLK4.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein tag; protein binding

Biological Process: nuclear mRNA splicing, via spliceosome; protein modification process