Rabbit anti-Human HUB1 Polyclonal Antibody | anti-HUB1 antibody

HUB1, CT (UBL5)

Cat. Num. AAA6010049

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HUB1; Polyclonal Antibody; CT (UBL5); Anti -HUB1; anti-HUB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-HUB1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry, ELISA; Dilution: ELISA1:1,000; Immunohistochemistry: 1:10-50
• Immunogen: HUB1(UBL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HUB1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

Related Product Information for anti-HUB1 antibody

UBL5 is a widely expressed human protein that is strongly conserved across phylogeny. Orthologs of UBL5 occur in every eukaryotic genome characterized to date. The yeast ortholog of UBL5, HUB1, was reported to be a ubiquitin-like protein modifier important for modulation of protein function. However, unlike ubiquitin and all other ubiquitin-like modifiers, UBL5 and its yeast ortholog HUB1 both contain a C-terminal di-tyrosine motif followed by a single variable residue instead of the characteristic di-glycine found in all other ubiquitin-like modifiers. Here we describe the three-dimensional structure of UBL5 determined by NMR. The overall structure of the protein was found to be very similar to ubiquitin despite the low approximately 25% residue similarity. The signature C-terminal di-tyrosine residues in UBL5 are involved in the final beta sheet of the protein. This is very different to the di-glycine motif found in ubiquitin, which extends beyond the final beta sheet. In addition, we have confirmed an earlier report of an interaction between UBL5 and the cyclin-like kinase, CLK4, which we have determined is specific and does not extend to other cyclin-like kinase family members.

Product Categories/Family for anti-HUB1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6324360
• NCBI GeneID: 855767
• NCBI Accession #: NP_014430.1
• NCBI GenBank Nucleotide #: NM_001184344.1
• UniProt Accession #: Q6Q546; D6W1K7
• Molecular Weight: 8,272 Da
• NCBI Official Full Name: Hub1p
• NCBI Official Symbol: HUB1
• NCBI Protein Information: Hub1p
• UniProt Protein Name: Ubiquitin-like modifier HUB1
• Protein Family: Ubiquitin-like modifier
• UniProt Gene Name: HUB1
• UniProt Entry Name: HUB1_YEAST

Uniprot Description

Function: Forms conjugate with SPH1 and HBT1. Involved in morphogenesis. Ref.4

Sequence similarities: Contains 1 ubiquitin-like domain.