Mouse HSP70/HSP72 Monoclonal Antibody | anti-HSP70/HSP72 antibody

HSP70/HSP72 monoclonal antibody (C92F3A-5)

Cat. Num. AAA565081

• Gene Names: HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
• Reactivity: Human, Mouse, Rat, Beluga, Bovine, C. elegans, Chicken, Dog, Drosophila, Fish, Gerbil, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Teal, Xenopus
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein G affinity purified.
• Synonyms: HSP70/HSP72; Monoclonal Antibody; HSP70/HSP72 monoclonal antibody (C92F3A-5); Hsp70; HspA1A; Heat shock protein 70; HspA1B; Hsp72; anti-HSP70/HSP72 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Beluga, Bovine, C. elegans, Chicken, Dog, Drosophila, Fish, Gerbil, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Teal, Xenopus
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C92F3A-5
• Specificity: Recognizes human, mouse, rat, bovine, beluga, C. elegans, dog, chicken, Drosophila, fish, gerbil, guinea pig, hamster, monkey, pig, rabbit, sheep, teal, and Xenopus HSP70 (HSP72). Detects a band of ~70kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 641

• Applicable Applications for anti-HSP70/HSP72 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), Electron Microscopy (EM)
• Application Notes: Immunohistochemistry (1:50); Western Blot (1:1'000, colorimetric).; Detects a band of ~70kDa by Western blot.
• Immunogen: Native human Hsp70 protein.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Testing Data

(Substrate binding domain of HSP70 in complex with a substrate peptide10.)

Related Product Information for anti-HSP70/HSP72 antibody

The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Product Categories/Family for anti-HSP70/HSP72 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 194248072
• NCBI GeneID: 3303
• NCBI Accession #: NP_005336.3
• NCBI GenBank Nucleotide #: NM_005345
• UniProt Accession #: P08107; P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0; B4E3B6; P0DMV8
• Molecular Weight: 63,937 Da
• NCBI Official Full Name: heat shock 70 kDa protein 1A
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 1A
• NCBI Official Symbol: HSPA1A
• NCBI Official Synonym Symbols: HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
• NCBI Protein Information: heat shock 70 kDa protein 1A
• UniProt Protein Name: Heat shock 70 kDa protein 1A
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSPA1; HSX70; HSP70.1
• UniProt Entry Name: HS71A_HUMAN

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Heat shock protein; Motility/polarity/chemotaxis; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: centriole; cytoplasm; cytosol; focal adhesion; inclusion body; mitochondrion; nucleoplasm; perinuclear region of cytoplasm; ubiquitin ligase complex

Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; histone deacetylase binding; protein binding; receptor binding; ubiquitin protein ligase binding; unfolded protein binding; viral receptor activity

Biological Process: activation of NF-kappaB transcription factor; ATP metabolic process; entry of virus into host cell; gene expression; negative regulation of protein ubiquitination; positive regulation of interleukin-8 production; protein refolding; protein stabilization; regulation of mRNA stability; regulation of protein ubiquitination