Rabbit DNAJA1 Polyclonal Antibody | anti-DNAJA1 antibody

DNAJA1 Polyclonal Antibody

Cat. Num. AAA2520477

• Gene Names: DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: DNAJA1; Polyclonal Antibody; DNAJA1 Polyclonal Antibody; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2; anti-DNAJA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.4mg/mL (varies by lot)
• Sequence Length: 397

• Applicable Applications for anti-DNAJA1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human DNAJA1
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of 231, A431, Raji, Jurkat and HepG2 cell using DNAJA1 Polyclonal Antibody at dilution of 1:500)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer using DNAJA1 Polyclonal Antibody at dilution of 1:30)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using DNAJA1 Polyclonal Antibody at dilution of 1:30)

Related Product Information for anti-DNAJA1 antibody

DnaJ-like proteins interact with HSP 70 molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 4504511
• NCBI GeneID: 3301
• NCBI Accession #: NP_001530.1
• NCBI GenBank Nucleotide #: NM_001539.2
• Molecular Weight: 37,045 Da
• NCBI Official Full Name: dnaJ homolog subfamily A member 1
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily A, member 1
• NCBI Official Symbol: DNAJA1
• NCBI Official Synonym Symbols: DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
• NCBI Protein Information: dnaJ homolog subfamily A member 1; HSJ-2; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein J2; heat shock protein, DNAJ-like 2; human DnaJ protein 2; neural precursor cell expressed, developmentally down-regulated 7
• UniProt Protein Name: DnaJ homolog subfamily A member 1
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJA1
• UniProt Synonym Gene Names: DNAJ2; HDJ2; HSJ2; HSPF4; HSJ-2; hDj-2
• UniProt Entry Name: DNJA1_HUMAN

Uniprot Description

DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.

Protein type: Chaperone; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: mitochondrion; membrane; perinuclear region of cytoplasm; cytosol; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; metal ion binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; Hsp70 protein binding; ATP binding

Biological Process: regulation of protein transport; negative regulation of JNK activity; sperm motility; protein folding; positive regulation of apoptosis; response to heat; androgen receptor signaling pathway; DNA damage response, detection of DNA damage; protein refolding; spermatogenesis; negative regulation of protein ubiquitination; response to unfolded protein; negative regulation of apoptosis