Rabbit anti-Human, Mouse HMGB3 Polyclonal Antibody | anti-HMGB3 antibody

HMGB3 Antibody

Cat. Num. AAA9403418

• Gene Names: HMGB3; HMG4; HMG-4; HMG2A; HMG-2a
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: HMGB3; Polyclonal Antibody; HMGB3 Antibody; high mobility group box 3; HMG4; HMG-4; HMG2A; HMG-2a; anti-HMGB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total HMGB3 protein.
• Form/Format: Supplied at 0.8mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 200

• Applicable Applications for anti-HMGB3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:10000; Western blotting: 1:2000-1:5000; Immunohistochemistry: 1:20-1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 164-178 amino acids of Human high mobility group box 3
• Target Name: HMGB3
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: 293T cell lysateLane2: Hela cell lysateLane3: Mouse lung tissue lysateLane4: Mouse brain tissue lysateLysates: 30ug per lanePrimary antibody: 1/400 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 1.5 minutes)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31220 (HMGB3 Antibody) at dilution 1/20, on the right is treated with the synthetic peptide.)

Related Product Information for anti-HMGB3 antibody

High-mobility group protein B3 is a protein that in humans is encoded by the HMGB3 gene. HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 and HMG2, HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription.

Product Categories/Family for anti-HMGB3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 71143137
• NCBI GeneID: 3149
• NCBI Accession #: NP_005333
• NCBI GenBank Nucleotide #: NM_005342.3
• Molecular Weight: 22,980 Da
• NCBI Official Full Name: high mobility group protein B3 isoform a
• NCBI Official Synonym Full Names: high mobility group box 3
• NCBI Official Symbol: HMGB3
• NCBI Official Synonym Symbols: HMG4; HMG-4; HMG2A; HMG-2a
• NCBI Protein Information: high mobility group protein B3
• UniProt Protein Name: High mobility group protein B3
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB3
• UniProt Synonym Gene Names: HMG2A; HMG4; HMG-2a; HMG-4
• UniProt Entry Name: HMGB3_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: chromosome; nucleus

Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity

Biological Process: chromatin remodeling; negative regulation of B cell differentiation; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination

Disease: Microphthalmia, Syndromic 13