HMGB3 recombinant protein

HMGB3, 1-180aa, Human, His tag, E Coli

Cat. Num. AAA205103

• Gene Names: HMGB3; HMG4; HMG-4; HMG2A; HMG-2a
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: HMGB3; 1-180aa; Human; His tag; E Coli; High mobility group protein B3; HMG-2a; HMG-4; HMG2A; HMG4; HMGB3 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAKGDPK KPKGKMSAYA FFVQTCREEH KKKNPEVPVN FAEFSKKCSE RWKTMSGKEK SKFDEMAKAD KVRYDREMKD YGPAKGGKKK KDPNAPKRPP SGFFLFCSEF RPKIKSTNPG ISIGDVAKKL GEMWNNLNDS EKQPYITKAA KLKEKYEKDV ADYKSKGKFD GAKGPAKVAR KKV
• Sequence Length: 200

• Applicable Applications for HMGB3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HMGB3 recombinant protein

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription. Recombinant human HMGB3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for HMGB3 recombinant protein

Epigenomics

References

Nemeth,M.J., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103 (37), 13783-13788; Ross,M.T., et al. (2005) Nature 434 (7031), 325-337

NCBI and Uniprot Product Information

• NCBI GI #: 71143137
• NCBI GeneID: 3149
• NCBI Accession #: NP_005333
• NCBI GenBank Nucleotide #: NM_005342.3
• UniProt Accession #: O15347; O95556; Q6NS40
• Molecular Weight: 22.8kDa (203aa) confirmed by MALDI-TOF
• NCBI Official Full Name: high mobility group protein B3 isoform a
• NCBI Official Synonym Full Names: high mobility group box 3
• NCBI Official Symbol: HMGB3
• NCBI Official Synonym Symbols: HMG4; HMG-4; HMG2A; HMG-2a
• NCBI Protein Information: high mobility group protein B3
• UniProt Protein Name: High mobility group protein B3
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB3
• UniProt Synonym Gene Names: HMG2A; HMG4; HMG-2a; HMG-4

NCBI Description

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling ().