Western Blot (WB)
(Western blot analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using MBS6001319 at 3ug/ml. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Rabbit HMG4 Polyclonal Antibody | anti-HMGB3 antibody
HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)
Gene Names
HMGB3; HMG4; HMG-4; HMG2A; HMG-2a
Reactivity
Bovine, Equine, Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
HMG4; Polyclonal Antibody; HMG4 (High Mobility Group 4 (HMG) Transcription Factor; High Mobility Group Protein 2a; HMG2a; High Mobility Group Box 3; HMGB3; MGC90319; Nonhistone Chromosomal Protein HMG4); Anti -HMG4 (High Mobility Group 4 (HMG) Transcription Factor; anti-HMGB3 antibody
Host
Rabbit
Reactivity
Bovine, Equine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human HMGB3. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Applicable Applications for anti-HMGB3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 3-5ug/ml
Dilution: Western Blot: 3-5ug/ml
Immunogen
Synthetic peptide corresponding to aa1-50 of human HMGB3.
Positive Control
Mouse placenta
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using MBS6001319 at 3ug/ml. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Western Blot (WB)
(Western blot analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using MBS6001319 at 3ug/ml. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.)
Related Product Information for anti-HMGB3 antibody
HMGB3, also known as High mobility group protein B3, is a DNA associated protein that binds preferentially single-stranded DNA and unwinds double stranded DNA. This transcription factor belongs to the HMGB family and high mobility group (HMG) protein superfamily and contains two HMG box DNA-binding domains. Like other members of the family, HMGB3 plays a fundamental role in DNA replication, nucleosome assembly and transcription. Other functions include regulation of balance between hematopoietic stem cell self-renewal and differentiation and may also be required during embryonic development. HMGB3 is predominantly expressed in placenta.
Product Categories/Family for anti-HMGB3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,980 Da
NCBI Official Full Name
high mobility group protein B3
NCBI Official Synonym Full Names
high mobility group box 3
NCBI Official Symbol
HMGB3
NCBI Official Synonym Symbols
HMG4; HMG-4; HMG2A; HMG-2a
NCBI Protein Information
high mobility group protein B3; high-mobility group box 3; high mobility group protein 4; high mobility group protein 2a; non-histone chromosomal protein; high-mobility group (nonhistone chromosomal) protein 4
UniProt Protein Name
High mobility group protein B3
Protein Family
UniProt Gene Name
HMGB3
UniProt Synonym Gene Names
HMG2A; HMG4; HMG-2a; HMG-4
UniProt Entry Name
HMGB3_HUMAN
NCBI Description
HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008]
Uniprot Description
HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: chromosome; nucleus
Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity
Biological Process: chromatin remodeling; negative regulation of B cell differentiation; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination
Disease: Microphthalmia, Syndromic 13
Research Articles on HMGB3
Similar Products
Product Notes
The HMGB3 hmgb3 (Catalog #AAA6001319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4) reacts with Bovine, Equine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HMG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 3-5ug/ml. Researchers should empirically determine the suitability of the HMGB3 hmgb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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