Western Blot (WB)
(Histone H3 phospho Thr45 antibody (pAb) tested by Western blot. Yeast extracts from a wild type strain (Lane 1) and a strain in which threonine 45 has been mutated to an alanine (T45A, Lane 2) were blotted and probed with Histone H3 phospho Thr45 antibody (pAb) at a dilution of 1:10,000. The arrow represents the migration of histone H3. Note that both the upper and lower bands disappear in the T45A mutant, suggesting that the upper band represents a post-translational modification of histone H3 that causes a dramatic decrease in gel mobility.)
Rabbit Histone H3T45ph Polyclonal Antibody | anti-H3T45ph antibody
Histone H3T45ph antibody (pAb)
Reactivity
Budding Yeast, Human, Wide Range Predicted
Applications
Dot Blot, Western Blot
Purity
Affinity Purified
Synonyms
Histone H3T45ph; Polyclonal Antibody; Histone H3T45ph antibody (pAb); anti-H3T45ph antibody
Host
Rabbit
Reactivity
Budding Yeast, Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3T45ph antibody
Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:2,000-1:10,000 dilution
This modification is extremely labile so non-denatured samples should not be allowed to sit in aqueous buffer. For Western blotting, overnight transfer at low voltage is recommended.
WB: 1:2,000-1:10,000 dilution
This modification is extremely labile so non-denatured samples should not be allowed to sit in aqueous buffer. For Western blotting, overnight transfer at low voltage is recommended.
Conjugation
Phosphorylated
Immunogen
This Histone H3 phospho Thr45 antibody was raised against a peptide containing phospho Thr45 of human histone H3.
Abs Residue
Thr
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone H3 phospho Thr45 antibody (pAb) tested by Western blot. Yeast extracts from a wild type strain (Lane 1) and a strain in which threonine 45 has been mutated to an alanine (T45A, Lane 2) were blotted and probed with Histone H3 phospho Thr45 antibody (pAb) at a dilution of 1:10,000. The arrow represents the migration of histone H3. Note that both the upper and lower bands disappear in the T45A mutant, suggesting that the upper band represents a post-translational modification of histone H3 that causes a dramatic decrease in gel mobility.)
Western Blot (WB)
(Histone H3 phospho Thr45 antibody (pAb) tested by Western blot. Yeast extracts from a wild type strain (Lane 1) and a strain in which threonine 45 has been mutated to an alanine (T45A, Lane 2) were blotted and probed with Histone H3 phospho Thr45 antibody (pAb) at a dilution of 1:10,000. The arrow represents the migration of histone H3. Note that both the upper and lower bands disappear in the T45A mutant, suggesting that the upper band represents a post-translational modification of histone H3 that causes a dramatic decrease in gel mobility.)
Dot Blot (DB)
(Histone H3 phospho Thr45 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr45 antibody (pAb) for Histone H3 phospho Thr45. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 phospho Thr45 antibody (pAb) at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row Lane 1: phospho Thr45 peptide. Lane 2: unmodified Thr45 peptide. Lane 3: phospho Thr3 peptide. Lane 4: phospho Thr6 peptide. Lane 5: phospho Thr11 peptide. Lane 6: phospho Thr80 peptide. Lane 7: phospho Ser10 peptide. Lane 8: phospho Ser28 peptide.)
Dot Blot (DB)
(Histone H3 phospho Thr45 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr45 antibody (pAb) for Histone H3 phospho Thr45. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 phospho Thr45 antibody (pAb) at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row Lane 1: phospho Thr45 peptide. Lane 2: unmodified Thr45 peptide. Lane 3: phospho Thr3 peptide. Lane 4: phospho Thr6 peptide. Lane 5: phospho Thr11 peptide. Lane 6: phospho Thr80 peptide. Lane 7: phospho Ser10 peptide. Lane 8: phospho Ser28 peptide.)
Related Product Information for anti-H3T45ph antibody
Short Description: Our Histone H3T45ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Budding Yeast and Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of histone H2A, histone H2B, histone H3 and histone H4). Histones are subject to a number of post-translational modifications, including acetylation, phosphorylation and methylation. In budding yeast, H3 Thr45 phosphorylation occurs specifically during DNA replication, and is catalyzed by the Cdc7-Dbf4 kinase. Yeast that lack threonine 45 exhibit defects in replication, suggesting that phosphorylation of Thr45 is associated with DNA replication. Threonine 45 lies within a conserved region of histone H3 that makes critical contacts with DNA when assembled into chromatin. It is located at the points of entry and exit of DNA on the nucleosome, and phosphorylation may influence histone-DNA interactions within the nucleosome.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of histone H2A, histone H2B, histone H3 and histone H4). Histones are subject to a number of post-translational modifications, including acetylation, phosphorylation and methylation. In budding yeast, H3 Thr45 phosphorylation occurs specifically during DNA replication, and is catalyzed by the Cdc7-Dbf4 kinase. Yeast that lack threonine 45 exhibit defects in replication, suggesting that phosphorylation of Thr45 is associated with DNA replication. Threonine 45 lies within a conserved region of histone H3 that makes critical contacts with DNA when assembled into chromatin. It is located at the points of entry and exit of DNA on the nucleosome, and phosphorylation may influence histone-DNA interactions within the nucleosome.
Product Categories/Family for anti-H3T45ph antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3T45ph (Catalog #AAA388560) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3T45ph antibody (pAb) reacts with Budding Yeast, Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3T45ph can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB). Applications Validated: WB: 1:2,000-1:10,000 dilution This modification is extremely labile so non-denatured samples should not be allowed to sit in aqueous buffer. For Western blotting, overnight transfer at low voltage is recommended. Researchers should empirically determine the suitability of the H3T45ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3T45ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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