Western Blot (WB)
(Histone H2AS129ph antibody (pAb) tested by Western blot. Whole-cell extract of yeast exposed to 200Gy ionizing radiation blotted with Histone H2A phospho Ser129 pAb at a dilution of 1:1,000. Lane 1: Yeast containing an alanine at position 129. Lane 2: Wild-type yeast.)
Rabbit Histone H2AS129ph Polyclonal Antibody | anti-H2AS129ph antibody
Histone H2AS129ph antibody (pAb)
Reactivity
Budding Yeast, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Synonyms
Histone H2AS129ph; Polyclonal Antibody; Histone H2AS129ph antibody (pAb); anti-H2AS129ph antibody
Host
Rabbit
Reactivity
Budding Yeast, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H2AS129ph antibody
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
WB: 1:500-1:2,000 dilution
Conjugation
Phosphorylated
Immunogen
This Histone H2A phospho Ser129 antibody was raised against a peptide including phospho-serine 129 of budding yeast Histone H2A.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone H2AS129ph antibody (pAb) tested by Western blot. Whole-cell extract of yeast exposed to 200Gy ionizing radiation blotted with Histone H2A phospho Ser129 pAb at a dilution of 1:1,000. Lane 1: Yeast containing an alanine at position 129. Lane 2: Wild-type yeast.)
Western Blot (WB)
(Histone H2AS129ph antibody (pAb) tested by Western blot. Whole-cell extract of yeast exposed to 200Gy ionizing radiation blotted with Histone H2A phospho Ser129 pAb at a dilution of 1:1,000. Lane 1: Yeast containing an alanine at position 129. Lane 2: Wild-type yeast.)
Dot Blot (DB)
(Histone H2AS129ph antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A phospho Ser129 pAb for phospho Ser129 histone H2A. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho-Ser129 peptide. Lane 2: Unmodified Ser129 peptide.)
Dot Blot (DB)
(Histone H2AS129ph antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A phospho Ser129 pAb for phospho Ser129 histone H2A. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho-Ser129 peptide. Lane 2: Unmodified Ser129 peptide.)
Related Product Information for anti-H2AS129ph antibody
Short Description: Our Histone H2AS129ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot and Western blot; it has been shown to react with Budding Yeast samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2A variant, H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2A at serine 129 is required for the efficient repair of DNA double-strand breaks.
Background: Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2A variant, H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2A at serine 129 is required for the efficient repair of DNA double-strand breaks.
Product Categories/Family for anti-H2AS129ph antibody
NCBI and Uniprot Product Information
Molecular Weight
14kDa
Similar Products
Product Notes
The H2AS129ph (Catalog #AAA388438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2AS129ph antibody (pAb) reacts with Budding Yeast, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2AS129ph can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the H2AS129ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2AS129ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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