Mouse Heat Shock 70kDa Protein 5 (HSPA5) CLIA Kit | HSPA5 clia kit

Mouse Heat Shock 70kDa Protein 5 (HSPA5) CLIA Kit

Cat. Num. AAA2709086

• Gene Names: Hspa5; Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Heat Shock 70kDa Protein 5 (HSPA5); Mouse Heat Shock 70kDa Protein 5 (HSPA5) CLIA Kit; Heat Shock 70kDa Protein 5; MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78; HSPA5 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5).; No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.
• Sequence Length: 655

• Applicable Applications for HSPA5 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.055ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heat Shock 70kDa Protein 5 (HSPA5) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 2506545
• NCBI GeneID: 14828
• UniProt Accession #: P20029
• NCBI Official Full Name: Endoplasmic reticulum chaperone BiP
• NCBI Official Synonym Full Names: heat shock protein 5
• NCBI Official Symbol: Hspa5
• NCBI Official Synonym Symbols: Bip; Sez7; mBiP; Grp78; SEZ-7; Hsce70; baffled; AL022860; AU019543; D2Wsu17e; D2Wsu141e
• NCBI Protein Information: endoplasmic reticulum chaperone BiP
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: Hspa5
• UniProt Synonym Gene Names: BiP

Uniprot Description

Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum (PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (PubMed:12411443).