Typical Testing Data/Standard Curve (for reference only)
Human Neutrophil Alkaline Phosphatase ELISA Kit | NAP elisa kit
Human Neutrophil Alkaline Phosphatase ELISA Kit
Gene Names
NAP1L1; NRP; NAP1; NAP1L
Reactivity
Human
Synonyms
Neutrophil Alkaline Phosphatase; Human Neutrophil Alkaline Phosphatase ELISA Kit; NAP elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This NAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NAP. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NAP elisa kit
Principle of the Assay: NAP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for NAP. Standards or samples are then added to the microtiter plate wells and NAP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of NAP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for NAP are added to each well to "sandwich" the NAP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NAP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42,762 Da
NCBI Official Full Name
NAP
NCBI Official Synonym Full Names
nucleosome assembly protein 1-like 1
NCBI Official Symbol
NAP1L1
NCBI Official Synonym Symbols
NRP; NAP1; NAP1L
NCBI Protein Information
nucleosome assembly protein 1-like 1; hNRP; NAP-1 related protein; NAP-1-related protein; HSP22-like protein interacting protein
UniProt Protein Name
Nucleosome assembly protein 1-like 1
UniProt Gene Name
NAP1L1
UniProt Synonym Gene Names
NRP; hNRP
UniProt Entry Name
NP1L1_HUMAN
NCBI Description
This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing of this gene results in several transcript variants; however, not all have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
NAP1L1: May be involved in modulating chromatin formation and contribute to regulation of cell proliferation. Belongs to the nucleosome assembly protein (NAP) family.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 12q21.2
Cellular Component: membrane; melanosome; nucleus
Molecular Function: protein binding
Biological Process: nucleosome assembly; positive regulation of cell proliferation; DNA replication
Research Articles on NAP
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Product Notes
The Human NAP nap1l1 (Catalog #AAA728894) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728894 ELISA Kit recognizes Human NAP. It is sometimes possible for the material contained within the vial of "Neutrophil Alkaline Phosphatase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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