Western Blot (WB)
(Histone H3.cs1 pAb tested by Western blot. Untreated (lane 1) and Cathepsin L-treated (lane 2 and 4) recombinant Histone H3.1 were blotted with Histone H3.cs1 pAb (top panel) or Histone H3 (bottom panel). Only the Cathepsin L-cleaved histone H3 is detected with the H3.cs1 antibody)
Rabbit anti-Human Histone H3.cs1 Polyclonal Antibody | anti-H3.cs1 antibody
Histone H3.cs1 antibody (pAb)
Reactivity
Human
Applications
Western Blot
Synonyms
Histone H3.cs1; Polyclonal Antibody; Histone H3.cs1 antibody (pAb); anti-H3.cs1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3.cs1 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution Depending upon the gel system employed, a change in mobility of the clipped form of histone H3 may not be observed.
WB: 1:500-1:1,000 dilution Depending upon the gel system employed, a change in mobility of the clipped form of histone H3 may not be observed.
Immunogen
This Histone H3.cs1 antibody was raised against a peptide containing the site of Cathepsin L cleavage at the N-terminus.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone H3.cs1 pAb tested by Western blot. Untreated (lane 1) and Cathepsin L-treated (lane 2 and 4) recombinant Histone H3.1 were blotted with Histone H3.cs1 pAb (top panel) or Histone H3 (bottom panel). Only the Cathepsin L-cleaved histone H3 is detected with the H3.cs1 antibody)
Western Blot (WB)
(Histone H3.cs1 pAb tested by Western blot. Untreated (lane 1) and Cathepsin L-treated (lane 2 and 4) recombinant Histone H3.1 were blotted with Histone H3.cs1 pAb (top panel) or Histone H3 (bottom panel). Only the Cathepsin L-cleaved histone H3 is detected with the H3.cs1 antibody)
Related Product Information for anti-H3.cs1 antibody
Short Description: Our Histone H3.cs1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone H3 is proteolytically cleaved at its N-terminus by Cathepsin L during the differentiation of embryonic stem cells. It is possible that this processing occurs in order to remove modifications that serve as protein recognition elements (e.g. Histone H3 trimethyl Lys9) present in the extreme N-terminus.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone H3 is proteolytically cleaved at its N-terminus by Cathepsin L during the differentiation of embryonic stem cells. It is possible that this processing occurs in order to remove modifications that serve as protein recognition elements (e.g. Histone H3 trimethyl Lys9) present in the extreme N-terminus.
Product Categories/Family for anti-H3.cs1 antibody
NCBI and Uniprot Product Information
Molecular Weight
15kDa
Similar Products
Product Notes
The H3.cs1 (Catalog #AAA388496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3.cs1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.cs1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution Depending upon the gel system employed, a change in mobility of the clipped form of histone H3 may not be observed. Researchers should empirically determine the suitability of the H3.cs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.cs1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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