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Chromatin Immunoprecipitation (ChIP)
(Histone H2B acetyl Lys16 pAb tested by ChIP analysis. ChIP performed on HeLa cell chromatin using 39121. PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: negative IgG control. Lane 2: ChIP using 10 ul of 39121. Lane 3: Input DNA control.)
Rabbit Histone H2BK16ac Polyclonal Antibody | anti-H2BK16ac antibody
Histone H2BK16ac antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Immunofluorescence, Western Blot
Synonyms
Histone H2BK16ac; Polyclonal Antibody; Histone H2BK16ac antibody (pAb); Histone H2B acetyl Lysine 16; anti-H2BK16ac antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H2BK16ac antibody
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 5-10ul per ChIP
WB: 1:5,000-1:20,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ChIP: 5-10ul per ChIP
WB: 1:5,000-1:20,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Conjugation
Acetylated
Immunogen
This Histone H2B acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of human histone H2B.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H2B acetyl Lys16 pAb tested by ChIP analysis. ChIP performed on HeLa cell chromatin using 39121. PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: negative IgG control. Lane 2: ChIP using 10 ul of 39121. Lane 3: Input DNA control.)
Chromatin Immunoprecipitation (ChIP)
(Histone H2B acetyl Lys16 pAb tested by ChIP analysis. ChIP performed on HeLa cell chromatin using 39121. PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: negative IgG control. Lane 2: ChIP using 10 ul of 39121. Lane 3: Input DNA control.)
Western Blot (WB)
(Histone H2B acetyl Lys16 pAb tested by Western blot. HeLa acid extract (5 ug per lane) was probed with Histone H2B acetyl Lys16 polyclonal antibody (1:20,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
Western Blot (WB)
(Histone H2B acetyl Lys16 pAb tested by Western blot. HeLa acid extract (5 ug per lane) was probed with Histone H2B acetyl Lys16 polyclonal antibody (1:20,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)
Dot Blot (DB)
(Histone H2B acetyl Lys16 pAb tested by dot blot analysis. Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys16 pAb for acetyl-Lys 16 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Lane 1: Peptide acetylated at lysine 5 of H2B. Lane 2: Unmodified lysine 5 peptide. Lane 3: Peptide acetylated at lysine 16 of H2B. Lane 4: unmodified lysine 16 peptide. Lane 5: Peptide acetylated at lysine 120 of H2B. Lane 6: Unmodified lysine 120 peptide.)
Dot Blot (DB)
(Histone H2B acetyl Lys16 pAb tested by dot blot analysis. Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys16 pAb for acetyl-Lys 16 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Lane 1: Peptide acetylated at lysine 5 of H2B. Lane 2: Unmodified lysine 5 peptide. Lane 3: Peptide acetylated at lysine 16 of H2B. Lane 4: unmodified lysine 16 peptide. Lane 5: Peptide acetylated at lysine 120 of H2B. Lane 6: Unmodified lysine 120 peptide.)
Immunofluorescence (IF)
(Detection of H2BK16ac by immunofluorescence U2OS cells were stained with H2BK16ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2BK16ac antibody staining. Right panel: merge.)
Immunofluorescence (IF)
(Detection of H2BK16ac by immunofluorescence U2OS cells were stained with H2BK16ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2BK16ac antibody staining. Right panel: merge.)
Related Product Information for anti-H2BK16ac antibody
Short Description: Our Histone H2BK16ac antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription. The acetylation of Histone H2B at Lys16 is associated with transcriptional activation and is involved in cell survival process.
Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription. The acetylation of Histone H2B at Lys16 is associated with transcriptional activation and is involved in cell survival process.
Product Categories/Family for anti-H2BK16ac antibody
NCBI and Uniprot Product Information
Molecular Weight
15kDa
Similar Products
Product Notes
The H2BK16ac (Catalog #AAA388376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2BK16ac antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2BK16ac can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ChIP: 5-10ul per ChIP WB: 1:5,000-1:20,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the H2BK16ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2BK16ac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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