Rabbit anti-Rat Histone Deacetylase 6 (HDAC6) Polyclonal Antibody | anti-HDAC6 antibody

Polyclonal Antibody to Histone Deacetylase 6 (HDAC6)

Cat. Num. AAA2005680

• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Histone Deacetylase 6 (HDAC6); Polyclonal Antibody; Polyclonal Antibody to Histone Deacetylase 6 (HDAC6); anti-HDAC6 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against HDAC6. It has been selected for its ability to recognize HDAC6 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GANFES IYICPSTFAC AQLATGAACR LVEAVLSGEV LNGIAIVRPP GHHAEPDAAC GFCFFNSVAV AARHAQVIAG RALRILIVDW DVHHGNGTQH IFEEDPSVLY VSLHRYDRGT FFPMGDEGAS SQVGRAAGTG FTVNVPWNGP RMGDADYLAT WHRLVLPIAY EFNPELVLIS AGFDAAQGDP LGGCQVTPEG YAHLTHLLMG LAGGRIILIL EGGYNLTSIS ESMAACTHSL LGDPPPQLTS LRP
• Sequence Length: 1215

• Applicable Applications for anti-HDAC6 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant HDAC6 (Gly565~Pro813) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2075745
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

• UniProt Accession #: F1LSE3
• Molecular Weight: 125,878 Da
• UniProt Protein Name: Histone deacetylase
• UniProt Gene Name: Hdac6

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: EC 3.5.1.98; Ubiquitin conjugating system; Deacetylase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: microtubule; leading edge; histone deacetylase complex; dendrite; perikaryon; inclusion body; caveola; cytosol; nucleoplasm; dynein complex; microtubule associated complex; axon; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasm; nucleus

Molecular Function: zinc ion binding; microtubule binding; histone deacetylase binding; beta-catenin binding; beta-tubulin binding; misfolded protein binding; Hsp90 protein binding; actin binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); tubulin deacetylase activity; ubiquitin protein ligase binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; polyubiquitin binding; tau protein binding; alpha-tubulin binding

Biological Process: negative regulation of proteolysis; response to misfolded protein; protein polyubiquitination; positive regulation of signal transduction; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; negative regulation of microtubule depolymerization; macroautophagy; regulation of fat cell differentiation; response to toxin; organelle organization and biogenesis; misfolded or incompletely synthesized protein catabolic process; histone deacetylation; regulation of gene expression, epigenetic; response to organic substance; intracellular protein transport; protein complex disassembly; protein amino acid deacetylation; lysosome localization; negative regulation of oxidoreductase activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent; negative regulation of protein complex disassembly

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia