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Western Blot (WB) (HIST1H2AK Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIST1H2AK antibody detected the HIST1H2AK protein (arrow).)

Rabbit anti-Human HIST1H2AK Polyclonal Antibody | anti-HIST1H2AK antibody

HIST1H2AK, NT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p) (PE)

Gene Names
HIST1H2AK; H2A/d; H2AFD
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HIST1H2AK; Polyclonal Antibody; NT (HIST1H2AG; H2AFP; Histone H2A type 1; Histone H2A/p) (PE); HIST1H2AG; Histone H2A/p; anti-HIST1H2AK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-HIST1H2AK antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
HIST1H2AK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2AK.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIST1H2AK Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIST1H2AK antibody detected the HIST1H2AK protein (arrow).)

Western Blot (WB) (HIST1H2AK Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HIST1H2AK antibody detected the HIST1H2AK protein (arrow).)
Related Product Information for anti-HIST1H2AK antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family.
Product Categories/Family for anti-HIST1H2AK antibody
References
Bergink, S., et al. Genes Dev. 20(10):1343-1352(2006). Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006). Boyne, M.T. II, et al. J. Proteome Res. 5(2):248-253(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,091 Da
NCBI Official Full Name
histone H2A type 1
NCBI Official Synonym Full Names
histone cluster 1, H2ak
NCBI Official Symbol
HIST1H2AK
NCBI Official Synonym Symbols
H2A/d; H2AFD
NCBI Protein Information
histone H2A type 1; H2A.1; histone 1, H2ak; histone H2A type 1-J; H2A histone family, member D
UniProt Protein Name
Histone H2A type 1
Protein Family
UniProt Gene Name
HIST1H2AG
UniProt Synonym Gene Names
H2AFP; H2A.1

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

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Product Notes

The HIST1H2AK hist1h2ag (Catalog #AAA6306768) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIST1H2AK, NT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIST1H2AK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HIST1H2AK hist1h2ag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIST1H2AK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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