TUBG1 recombinant protein

TUBG1, 1-451aa, Human, His-tag, Baculovirus

Cat. Num. AAA206816

• Gene Names: TUBG1; TUBG; GCP-1; CDCBM4; TUBGCP1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: TUBG1; 1-451aa; Human; His-tag; Baculovirus; Tubulin gamma-1 chain; CDCBM4; GCP-1; TUBG; TUBGCP1; TUBG1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 40% glycerol, 0.1M NaCl, 2mM DTT, 50mM imidazole.
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE QHHHHHH
• Sequence Length: 451

• Applicable Applications for TUBG1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TUBG1 recombinant protein

TUBG1, also known as tubulin gamma-1 chain, is a member of the tubulin superfamily. This protein is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. It is the pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. It is required for microtubule formation and progression of the cell cycle. Also, this protein is interacts with GCP2, GCP3, and B9D2. The interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Recombinant human TUBG1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TUBG1 recombinant protein

Signal Transduction; Cytoskeleton

References

Zheng Y., et al, (1991) Cell 65:817-823. Kapeller R., et al, (1995) J. Biol. Chem. 270:25985-25991.

NCBI and Uniprot Product Information

• NCBI GI #: 31543831
• NCBI GeneID: 7283
• NCBI Accession #: NP_001061
• NCBI GenBank Nucleotide #: NM_001070.4
• UniProt Accession #: P23258; Q53X79; Q9BW59
• Molecular Weight: 51.9kDa (457aa) 50-70kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: tubulin gamma-1 chain
• NCBI Official Synonym Full Names: tubulin gamma 1
• NCBI Official Symbol: TUBG1
• NCBI Official Synonym Symbols: TUBG; GCP-1; CDCBM4; TUBGCP1
• NCBI Protein Information: tubulin gamma-1 chain
• UniProt Protein Name: Tubulin gamma-1 chain
• UniProt Gene Name: TUBG1
• UniProt Synonym Gene Names: TUBG; GCP-1

NCBI Description

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]

Uniprot Description

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.