Rabbit anti-Human COQ7 Polyclonal Antibody | anti-COQ7 antibody

COQ7, CT (COQ7, Ubiquinone biosynthesis protein COQ7 homolog, Coenzyme Q biosynthesis protein 7 homolog, Timing protein clk-1 homolog) (Azide free) (HRP)

Cat. Num. AAA6287959

• Gene Names: COQ7; CAT5; CLK1; CLK-1; COQ10D8
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COQ7; Polyclonal Antibody; CT (COQ7; Ubiquinone biosynthesis protein COQ7 homolog; Coenzyme Q biosynthesis protein 7 homolog; Timing protein clk-1 homolog) (Azide free) (HRP); Timing protein clk-1 homolog; anti-COQ7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-COQ7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: COQ7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-199 amino acids from the C-terminal region of human COQ7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COQ7 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the COQ7 antibody detected the COQ7 protein (arrow).)

Immunohistochemistry (IHC)

(COQ7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COQ7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(COQ7 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-COQ7 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 25453484
• NCBI GeneID: 10229
• NCBI Accession #: NP_057222.2; NP_001177912.1
• NCBI GenBank Nucleotide #: NM_016138.4
• UniProt Accession #: Q99807; Q9BTT7; Q9H0T5; Q9UEW5; Q9UNR5; B2RDA9
• Molecular Weight: 20,158 Da
• NCBI Official Full Name: 5-demethoxyubiquinone hydroxylase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: coenzyme Q7, hydroxylase
• NCBI Official Symbol: COQ7
• NCBI Official Synonym Symbols: CAT5; CLK1; CLK-1; COQ10D8
• NCBI Protein Information: 5-demethoxyubiquinone hydroxylase, mitochondrial
• UniProt Protein Name: Ubiquinone biosynthesis protein COQ7 homolog
• Protein Family: 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
• UniProt Gene Name: COQ7
• UniProt Entry Name: COQ7_HUMAN

NCBI Description

The protein encoded by this gene is similar to a mitochondrial di-iron containing hydroxylase in Saccharomyces cerevisiae that is involved with ubiquinone biosynthesis. Mutations in the yeast gene lead to slower development and longer life span. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

COQ7: Involved in lifespan determination in ubiquinone- independent manner. Involved in ubiquinone biosynthesis. Potential central metabolic regulator. Belongs to the COQ7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Mitochondrial

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: mitochondrial inner membrane; nucleus

Molecular Function: metal ion binding

Biological Process: ubiquinone biosynthetic process; neurogenesis; in utero embryonic development; determination of adult life span; neural tube formation; age-dependent response to oxidative stress; organelle ATP synthesis coupled electron transport