Rabbit anti-Mouse TCF7L2 Polyclonal Antibody | anti-TCF7L2 antibody

TCF7L2, NT (TCF7L2, TCF4, Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4) (PE)

Cat. Num. AAA6354523

• Gene Names: TCF7L2; TCF4; TCF-4
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TCF7L2; Polyclonal Antibody; NT (TCF7L2; TCF4; Transcription factor 7-like 2; HMG box transcription factor 4; T-cell-specific transcription factor 4) (PE); T-cell-specific transcription factor 4; anti-TCF7L2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 455

• Applicable Applications for anti-TCF7L2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TCF7L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human TCF7L2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TCF7L2 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TCF7L2 antibody detected the TCF7L2 protein (arrow).)

Related Product Information for anti-TCF7L2 antibody

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.

Product Categories/Family for anti-TCF7L2 antibody

Antibodies; Transcription Factors

References

Hansson, O., et al. Curr. Diab. Rep. 10(6):444-451(2010). Heni, M., et al. Diabetes (2010). In press: Potapov, V.A., et al. Genetika 46(8):1123-1131(2010). Kucharska-Newton, A.M., et al. J Obes 2010 (2010): Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 226371623
• NCBI GeneID: 6934
• NCBI Accession #: NP_001139756.1; NP_001139746.1
• NCBI GenBank Nucleotide #: NP_001139756.1
• UniProt Accession #: Q9NQB0
• NCBI Official Full Name: transcription factor 7-like 2 isoform 4
• NCBI Official Synonym Full Names: transcription factor 7 like 2
• NCBI Official Symbol: TCF7L2
• NCBI Official Synonym Symbols: TCF4; TCF-4
• NCBI Protein Information: transcription factor 7-like 2
• UniProt Protein Name: Transcription factor 7-like 2
• Protein Family: Transcription factor
• UniProt Gene Name: TCF7L2
• UniProt Synonym Gene Names: TCF4; T-cell factor 4; TCF-4; hTCF-4
• UniProt Entry Name: TF7L2_HUMAN

NCBI Description

This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

Uniprot Description

TCF7L2: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. Interacts with TGFB1I1. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. Belongs to the TCF/LEF family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Motility/polarity/chemotaxis; DNA-binding; Transcription factor; Cell development/differentiation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: nucleoplasm; transcription factor complex; PML body; cytoplasm; nuclear chromatin; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; gamma-catenin binding; beta-catenin binding; chromatin binding; transcription factor binding; transcription factor activity; protein kinase binding; nuclear hormone receptor binding

Biological Process: neural tube development; skin development; glycogen metabolic process; fat cell differentiation; regulation of myelination; somatic stem cell maintenance; positive regulation of apoptosis; Wnt receptor signaling pathway through beta-catenin; glucose homeostasis; maintenance of DNA repeat elements; negative regulation of BMP signaling pathway; post-embryonic development; embryonic digestive tract morphogenesis; response to glucose stimulus; cell cycle arrest; oligodendrocyte development; embryonic hindgut morphogenesis; embryonic genitalia morphogenesis; regulation of hormone metabolic process; transcription, DNA-dependent; regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; glucose metabolic process; positive regulation of insulin secretion; cellular response to starvation; negative regulation of organ growth; negative regulation of fat cell differentiation; regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; secretory granule localization; myoblast cell fate commitment; positive regulation of protein binding; negative regulation of fibroblast growth factor receptor signaling pathway; regulation of oligodendrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; bone mineralization; positive regulation of protein export from nucleus; positive regulation of gluconeogenesis; pancreas development; blood vessel development; multicellular organism growth; odontogenesis of dentine-containing teeth; positive regulation of protein kinase B signaling cascade; cell proliferation; generation of neurons; pituitary gland development; regulation of skeletal muscle development; brain development

Disease: Diabetes Mellitus, Noninsulin-dependent