Western Blot (WB)
(Sample Data: Representative western blot (1:1000) using in a positive multi-tag cell lysate that contains a 52 kDa His tagged fusion protein.)
Rabbit His Polyclonal Antibody | anti-His antibody
Anti-His
Reactivity
All proteins tagged with 6X His
Applications
ELISA, Immunoprecipitation
Purity
Purified by antigen-specific affinity chromatography
Synonyms
His; Polyclonal Antibody; Anti-His; anti-His antibody
Host
Rabbit
Reactivity
All proteins tagged with 6X His
Clonality
Polyclonal
Specificity
Anti-His antibody recognizes the N-terminal, C-terminal or internal 6X His-tagged fusion protein
Purity/Purification
Purified by antigen-specific affinity chromatography
Form/Format
TBS pH7.2, 50% glycerol
Applicable Applications for anti-His antibody
ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 21640:22639-Immunostaining
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) using in a positive multi-tag cell lysate that contains a 52 kDa His tagged fusion protein.)
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) using in a positive multi-tag cell lysate that contains a 52 kDa His tagged fusion protein.)
Related Product Information for anti-His antibody
This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally [1]. The 6 His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state [2].
Product Categories/Family for anti-His antibody
References
1. Jonathan, W. et al: Epitope tagging, Annual Review of Genetics, 1998; 32, 601-618
2. Schmeisser, H, et al:, Binding Characteristics of IFN-alpha Subvariants to IFNAR2-EC and Influence of the 6-Histidine Tag. J Interferon Cytokine Res. 2006; Dec. 26(12):866-76.
2. Schmeisser, H, et al:, Binding Characteristics of IFN-alpha Subvariants to IFNAR2-EC and Influence of the 6-Histidine Tag. J Interferon Cytokine Res. 2006; Dec. 26(12):866-76.
Similar Products
Product Notes
The His (Catalog #AAA516012) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-His reacts with All proteins tagged with 6X His and may cross-react with other species as described in the data sheet. AAA Biotech's His can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP). WB: 21640:22639-Immunostaining. Researchers should empirically determine the suitability of the His for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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