Rabbit anti-Mouse, Rat NR2A Polyclonal Antibody | anti-NR2A antibody

NR2A (Grin2a, Glutamate Receptor, ionotropic, NMDA2A (epsilon 1), GluRepsilon1, NMDAR2A, N-methyl D-aspartate Receptor subtype 2A) (APC)

Cat. Num. AAA6490486

• Gene Names: Grin2a; NR2A; GluN2A; NMDAR2A; GluRepsilon1
• Reactivity: Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR2A; Polyclonal Antibody; NR2A (Grin2a; Glutamate Receptor; ionotropic; NMDA2A (epsilon 1); GluRepsilon1; NMDAR2A; N-methyl D-aspartate Receptor subtype 2A) (APC); anti-NR2A antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse NR2A at ~170kD. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 1464

• Applicable Applications for anti-NR2A antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 4ug immunoprecipitates NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.; Applications are based on unconjugated antibody.; WB: 0.5-2ug/ml detects NR2A (~170kD) in rat brain microsomal preparations. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with.
• Immunogen: 6 His-tagged fusion protein corresponding to aa1265-1464 of mouse NMDA receptor 2A (NR2A) (NP_032196).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: A rat brain microsomal preparation was probed with N5380-25, (0.5ug/ml). Arrow indicates NR2A (~170kD).)

Product Categories/Family for anti-NR2A antibody

Antibodies; Ion Channel

References

1. Bliss, T.V.P. & Collingridge, G.L., (1993) Nature 361:31-39. 2. Choi, D.W. & Rothman, S.M., (1990) Ann. Rev. Neurosci. 13:171-182. 3. Constantine-Patton, M., et al., (1990) Ann. Rev. Nerosci. 13:129-154. 4. Mandich, P., et al., (1994) Genomics 22:216-218. 5. Yoshii, A., et al. (2003). PNAS 100:1334-1339. 6. Bleich, S., et al., (2003). Int. J. Geriatr. Psychiatry 18:S33-S40. 7. Mori, H. & Mishina, M., (2003). Life Sci. 74:329-336. 8. Mayer, M.L. & Armstrong, N., (2004). Ann. Rev. Physiol. 66:161-181. 9. Lau, L.F., et al., (1996). J. Biol. Chem. 271:21, 622-21, 628. 10. Muller, B.M., et al., (1996). Neuron 17:255-265. 11. Tabuchi, Katsuhiko, et al. (2007). Science, 318: 71-6 (2007). 12. Molinuevo, Jose L, et al. (2005). American journal of Alzheimer's disease and other dementias, 20: 77-85 (2005). 13. Herin, Greta Ann and Aizenman, Elias (2004). Eur. J. Pharmacol., 500: 101-11 (2004). 14. Mayer, Mark L and Armstrong, Neali (2004). Annu. Rev. Physiol., 66: 161-81 (2004). 15. Popescu, Gabriela and Auerbach, Anthony (2004). The Neuroscientist: a review journal bringing neurobiology, neurology and psychiatry, 10: 192-8 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 41680705
• NCBI GeneID: 14811
• NCBI Accession #: NP_032196
• NCBI GenBank Nucleotide #: NP_032196.2
• UniProt Accession #: Q12879; P35436
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, NMDA2A (epsilon 1)
• NCBI Official Symbol: Grin2a
• NCBI Official Synonym Symbols: NR2A; GluN2A; NMDAR2A; GluRepsilon1
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• UniProt Gene Name: GRIN2A
• UniProt Synonym Gene Names: NMDAR2A; GluN2A; NMDAR2A; NR2A; hNR2A
• UniProt Entry Name: NMDE1_HUMAN

Uniprot Description

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity

Biological Process: startle response; positive regulation of apoptosis; regulation of synaptic plasticity; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; response to wounding; visual learning; serotonin metabolic process; response to drug; synaptic transmission, glutamatergic; glutamate signaling pathway; response to amphetamine; sleep; memory; response to ethanol; neurogenesis; directional locomotion; ionotropic glutamate receptor signaling pathway; regulation of sensory perception of pain; negative regulation of protein catabolic process; regulation of excitatory postsynaptic membrane potential

Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation