Western Blot (WB)
(Western blot analysis of His-Tag Rabbit pAb expression in His fusion protein sample)
Rabbit anti-All His-Tag Polyclonal Antibody
His-Tag Rabbit pAb
Reactivity
All
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
His-Tag; Polyclonal Antibody; His-Tag Rabbit pAb; anti-His-Tag antibody
Host
Rabbit
Reactivity
All
Clonality
Polyclonal
Specificity
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol.
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-His-Tag antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:3000-1:10000
Immunogen
Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
Subcellular Location
Endoplasmic Reticulum Membrane. Microsome Membrane.
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of His-Tag Rabbit pAb expression in His fusion protein sample)
Western Blot (WB)
(Western blot analysis of His-Tag Rabbit pAb expression in His fusion protein sample)
Related Product Information for anti-His-Tag antibody
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
References
Shahzad T, Zhan MY, Yang PJ, Yu XQ, Rao XJ; Journal: Arch Insect Biochem Physiol. Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori. Cao X, Yu H, Chen C, Wei J, Wang P; Journal: Biotechnol Lett. Expression and characterization of recombinant humanized anti-HER2 single-chain antibody in Pichia pastoris for targeted cancer therapy. Rao XJ, Wu P, Shahzad T, Liu S, Chen L, Yang YF, Shi Q, Yu XQ; Journal: Dev Comp Immunol. Characterization of a dual-CRD galectin in the silkworm Bombyx mori. Zhan MY, Shahzad T, Yang PJ, Liu S, Yu XQ, Rao XJ; Journal: Dev Comp Immunol. A single-CRD C-type lectin is important for bacterial clearance in the silkworm.
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Product Notes
The His-Tag (Catalog #AAA9610332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The His-Tag Rabbit pAb reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:3000-1:10000. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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