Western Blot (WB)
(Sample Data: Representative western blot (1:1000) using in a positive cell lysate that contains V5 tagged fusion protein.)
Mouse V5 Monoclonal Antibody | anti-V5 antibody
Anti-V5
Reactivity
All proteins tagged with V5
Applications
ELISA, Immunoprecipitation
Purity
Purified by Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Synonyms
V5; Monoclonal Antibody; Anti-V5; anti-V5 antibody
Host
Mouse
Reactivity
All proteins tagged with V5
Clonality
Monoclonal
Isotype
Mouse, IgG1
Clone Number
V4RR
Specificity
Anti-V5 antibody recognizes the N-terminal or C-terminal V5-tagged (GKPIPNPLLGLDST sequence) fusion protein.
Purity/Purification
Purified by Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Form/Format
10mM PBS pH7.2
Applicable Applications for anti-V5 antibody
ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 21598:22597-Immunostaining
Immunogen
GKPIPNPLLGLDST peptide conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) using in a positive cell lysate that contains V5 tagged fusion protein.)
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) using in a positive cell lysate that contains V5 tagged fusion protein.)
Related Product Information for anti-V5 antibody
The V5 epitope tag (GKPIPNPLLGLDST) consists of 14 highly charged amino acid residues originating from the C-terminus end of the P and V proteins of the simian virus 5, of the paramyxovirus family [1]. This tag is widely employed because of the highly specific antibodies that can be generated against it [2].
Product Categories/Family for anti-V5 antibody
References
1. Southern, J.A. et al., Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics, J. Gen. Virol. 72, 1991; (7), pp. 1551-1557.
2. Thomas, M, et al., Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43, J. of Virol. Methods Vol. 139, (2), Feb. 2007; pp. 175-180.
2. Thomas, M, et al., Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43, J. of Virol. Methods Vol. 139, (2), Feb. 2007; pp. 175-180.
Similar Products
Product Notes
The V5 (Catalog #AAA516296) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-V5 reacts with All proteins tagged with V5 and may cross-react with other species as described in the data sheet. AAA Biotech's V5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP). WB: 21598:22597-Immunostaining. Researchers should empirically determine the suitability of the V5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "V5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
