Western Blot (WB)
(Western blot analysis of HFE expression in mouse heart (A), mouse liver (B) whole cell lysates.)
Rabbit anti-Human HFE Polyclonal Antibody | anti-HFE antibody
Anti-HFE Antibody
Western Blot (WB)
(Western blot analysis of HFE expression in mouse heart (A), mouse liver (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of HFE expression in mouse heart (A), mouse liver (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HFE staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HFE staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
HFE: Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. Defects in HFE are a cause of hemochromatosis (HFE). A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading. Defects in HFE are associated with variegate porphyria (VP). Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria. Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the MHC class I family. 11 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: apical part of cell; cytoplasmic vesicle; early endosome; external side of plasma membrane; extracellular space; integral to plasma membrane; MHC class I protein complex; perinuclear region of cytoplasm; plasma membrane; recycling endosome
Molecular Function: beta-2-microglobulin binding; peptide antigen binding; protein binding; receptor binding
Biological Process: acute-phase response; antigen processing and presentation; antigen processing and presentation of peptide antigen via MHC class I; BMP signaling pathway; cellular iron ion homeostasis; cellular response to iron ion starvation; female pregnancy; hormone biosynthetic process; immune response; iron ion homeostasis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of T cell antigen processing and presentation; negative regulation of T cell cytokine production; positive regulation of protein binding; positive regulation of receptor-mediated endocytosis; protein complex assembly; response to iron ion
Disease: Alzheimer Disease; Hemochromatosis, Type 1; Microvascular Complications Of Diabetes, Susceptibility To, 7; Porphyria Cutanea Tarda; Porphyria Variegata; Transferrin Serum Level Quantitative Trait Locus 2
Research Articles on HFE
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Product Notes
The HFE hfe (Catalog #AAA8245572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HFE Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HFE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the HFE hfe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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