Rabbit anti-Human, Mouse TAR DNA-binding protein 43 Polyclonal Antibody | anti-TARDBP antibody

Rabbit anti-human TAR DNA-binding protein 43 polyclonal Antibody(TARDBP)

Cat. Num. AAA1497439

• Gene Names: TARDBP; ALS10; TDP-43
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: TAR DNA-binding protein 43; Polyclonal Antibody; Rabbit anti-human TAR DNA-binding protein 43 polyclonal Antibody(TARDBP); TDP-43; TARDBP; TDP43; anti-TARDBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 414

• Applicable Applications for anti-TARDBP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-5000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human TARDBP protein (1-260aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-102127 / sc-292299
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: TAR DNA-binding protein 43 antibody at 8ug/mlLane 1:Hela whole cell lysateLane 2:mouse spleen tissueLane:K562 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45,32 kDaObserved band size: 45 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1497439 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta using MBS1497439 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating TARDBP in Hela whole cell lysateLane 1: Rabbit control IgG (1ug)instead of MBS1497439 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS1497439 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))

Related Product Information for anti-TARDBP antibody

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

NCBI and Uniprot Product Information

• NCBI GI #: 6678271
• NCBI GeneID: 23435
• NCBI Accession #: NP_031401.1
• NCBI GenBank Nucleotide #: NM_007375.3
• UniProt Accession #: Q13148; Q53H27; Q6FI92; Q96DJ0; A4GUK4; A4GUK5; A4GUK6; B2R629; B4DJ45; E2PU12
• Molecular Weight: 31,808 Da
• NCBI Official Full Name: TAR DNA-binding protein 43
• NCBI Official Synonym Full Names: TAR DNA binding protein
• NCBI Official Symbol: TARDBP
• NCBI Official Synonym Symbols: ALS10; TDP-43
• NCBI Protein Information: TAR DNA-binding protein 43
• UniProt Protein Name: TAR DNA-binding protein 43
• Protein Family: TAR DNA-binding protein
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43
• UniProt Entry Name: TADBP_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

Uniprot Description

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytoplasm; nuclear speck; nucleoplasm; nucleus; perichromatin fibrils

Molecular Function: double-stranded DNA binding; identical protein binding; mRNA 3'-UTR binding; nucleotide binding; protein binding; RNA binding; transcription factor activity

Biological Process: mRNA processing; negative regulation of protein amino acid phosphorylation; nuclear fragmentation during apoptosis; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; RNA splicing; transcription from RNA polymerase II promoter

Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia