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Western Blot (WB) (Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SRPRB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Signal Recognition Particle Receptor B Polyclonal Antibody | anti-SRPRB antibody

Signal Recognition Particle Receptor B (SRPRB) Polyclonal Antibody

Gene Names
SRPRB; APMCF1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Signal Recognition Particle Receptor B; Polyclonal Antibody; Signal Recognition Particle Receptor B (SRPRB) Polyclonal Antibody; APMCF1; anti-SRPRB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SRPRB. It has been selected for its ability to recognize SRPRB in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
264
Applicable Applications for anti-SRPRB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Pig
Organism Species
Human
Fragment
SRPRB (Arg58~Ala271)
Quality Control
Content: The quality control contains recombinant SRPRB (Arg58~Ala271) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2068691
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SRPRB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SRPRB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,702 Da
NCBI Official Full Name
signal recognition particle receptor subunit beta
NCBI Official Synonym Full Names
SRP receptor beta subunit
NCBI Official Symbol
SRPRB
NCBI Official Synonym Symbols
APMCF1
NCBI Protein Information
signal recognition particle receptor subunit beta
UniProt Protein Name
Signal recognition particle receptor subunit beta
UniProt Gene Name
SRPRB
UniProt Synonym Gene Names
SR-beta

NCBI Description

The protein encoded by this gene has similarity to mouse protein which is a subunit of the signal recognition particle receptor (SR). This subunit is a transmembrane GTPase belonging to the GTPase superfamily. It anchors alpha subunit, a peripheral membrane GTPase, to the ER membrane. SR is required for the cotranslational targeting of both secretory and membrane proteins to the ER membrane. [provided by RefSeq, Jul 2008]

Uniprot Description

SRPRB: Component of the SRP (signal recognition particle) receptor. Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system. Has GTPase activity. May mediate the membrane association of SRPR. Belongs to the SRP receptor beta subunit family.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: cytoplasm; cytoplasmic microtubule; endoplasmic reticulum membrane; integral component of membrane; membrane; signal recognition particle receptor complex

Molecular Function: GTP binding; signal recognition particle binding

Biological Process: small GTPase mediated signal transduction

Research Articles on SRPRB

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Product Notes

The SRPRB srprb (Catalog #AAA2032763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Signal Recognition Particle Receptor B (SRPRB) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Signal Recognition Particle Receptor B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SRPRB srprb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Signal Recognition Particle Receptor B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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