Angiotensin I Converting Enzyme 2 (ACE2) Recombinant Protein | ACE2 recombinant protein

Recombinant Angiotensin I Converting Enzyme 2 (ACE2)

Cat. Num. AAA2125513

• Gene Names: Ace2; 2010305L05Rik
• Applications: SDS-Page, Western Blot
• Purity: Greater than 90% by SDS-PAGE
• Synonyms: Angiotensin I Converting Enzyme 2 (ACE2); Recombinant Angiotensin I Converting Enzyme 2 (ACE2); ACE2/Angiotensin I Converting Enzyme 2; ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; ACE2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% by SDS-PAGE
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Gln18~Thr740

• Applicable Applications for ACE2 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Tag: N-terminal His Tag
• Expression System: Prokaryotic expression
• Organism Species: Mouse
• Isoelectric Point: 5.2
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

Gene Sequencing

(Gene Sequencing (extract))

SDS-Page

(SDS-PAGE)

Product Categories/Family for ACE2 recombinant protein

Enzyme & Kinase; Metabolic pathway; Endocrinology; Cardiovascular biology; Neuro science; Hepatology; Prokaryotic recombinant

NCBI and Uniprot Product Information

• NCBI GI #: 71152217
• NCBI GeneID: 70008
• UniProt Accession #: Q8R0I0; Q99N70; Q99N71
• Molecular Weight: 40,340 Da
• NCBI Official Full Name: Angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• NCBI Official Symbol: Ace2
• NCBI Official Synonym Symbols: 2010305L05Rik
• NCBI Protein Information: angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: Ace2
• UniProt Entry Name: ACE2_MOUSE

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.23; Protease; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: viral receptor activity; peptidase activity; peptidyl-dipeptidase activity; hydrolase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; negative regulation of smooth muscle cell proliferation; receptor biosynthetic process; proteolysis; angiotensin mediated drinking behavior; regulation of systemic arterial blood pressure by renin-angiotensin