Rabbit HDAC2 Polyclonal Antibody | anti-phospho-HDAC2 antibody

Anti-phospho-HDAC2 (Ser394)

Cat. Num. AAA515999

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Affinity chromatography
• Synonyms: HDAC2; Polyclonal Antibody; Anti-phospho-HDAC2 (Ser394); anti-phospho-HDAC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit, IgG
• Specificity: Recognizes the HDAC2 protein phosphorylated at serine 394
• Purity/Purification: Affinity chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-phospho-HDAC2 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
• Immunogen: Phosphopeptide derived from human HDAC2 around the phosphorylation site of serine 394 (E-D-SP-G-D).
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; 1 year at -20 degree C from date of shipment

Immunohistochemistry (IHC)

(Sample Data: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-HDAC2 (Ser394).)

Western Blot (WB)

(Sample Data: Western blot analysis of extracts from HT-29 cells untreated or treated with UV (20 mins), using HDAC2 antibody and Anti-phospho-HDAC2 (Ser394) (Lane 3 and 4))

Related Product Information for anti-phospho-HDAC2 antibody

HDAC2 or Histone deacetylase 2 belongs to the histone deacetylase family that acts via the formation of large multiprotein complexes, and is responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). HDAC2 forms transcriptional repressor complexes by associating with many different proteins and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC2 functions in modulating synaptic plasticity and long-lasting changes of neural circuits, which in turn negatively regulates learning and memory (1). HDAC1 and HDAC2 are functionally redundant in cardiac growth and development and they maintain cardiomyocyte identity and function (2).

Product Categories/Family for anti-phospho-HDAC2 antibody

Phospho Specific Antibodies; Cancer; Cardiovascular Disease; Cell Cycle; Inflammation; WNT Signaling

References

1. Guan, J.S. et.al: HDAC2 negatively regulates memory formation and synaptic plasticity. Nature 459: 55-60, 2009.
2. Montgomery, R. L. et.al: Histone deacetylases 1 and 2 redundantly regulate cardiac morphogenesis, growth, and contractility. Genes Dev. 21: 1790-1802, 2007.