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Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using HDAC2 antibody)

Rabbit HDAC2 Polyclonal Antibody | anti-HDAC2 antibody

HDAC2 antibody

Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
HDAC2; Polyclonal Antibody; HDAC2 antibody; Rabbit polyclonal HDAC2 antibody; Polyclonal HDAC2 antibody; Anti-HDAC2 antibody; HDAC-2 antibody; HDAC-2; YAF1 antibody; HDAC 2; RPD3 antibody; Histone deacetylase 2 antibody; Ab 394 antibody; HDAC 2 antibody; anti-HDAC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-HDAC2 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
HDAC2 antibody was raised in Rabbit using the peptide sequence around aa 392~396 (E-D-S-G-D) derived from Human HDAC2 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using HDAC2 antibody)

Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using HDAC2 antibody)

Western Blot (WB)

(Western Blot analysis of extracts from 293 and Hela cells using HDAC2 antibody)

Western Blot (WB) (Western Blot analysis of extracts from 293 and Hela cells using HDAC2 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 antibody (left) or the same antibody preincubated with with blocking peptide (right))

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2 antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-HDAC2 antibody
HDAC2 belongs to the histone deacetylase family that act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones. It forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. It also plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Product Categories/Family for anti-HDAC2 antibody

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Product Notes

The HDAC2 (Catalog #AAA5309724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HDAC2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HDAC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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