Rabbit Hairless Polyclonal Antibody | anti-E77 antibody

Hairless (E77) Polyclonal Antibody

Cat. Num. AAA3013432

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Hairless; Polyclonal Antibody; Hairless (E77) Polyclonal Antibody; Lysine-specific demethylase hairless; HR; CG5460; CT36411; CT17282; anti-E77 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Hairless (E77) polyclonal antibody detects endogenous levels of Hairless protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1189

• Applicable Applications for anti-E77 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human Hairless.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HR pAb at 1:500 dilutionLane1:The Small intestine tissue lysate of Rat (40ug) Lane2:A375 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Hairless (E77) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-E77 antibody

Drosophila genes can be categorized based on the type of protein for which they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) or other functional proteins. Among these numerous proteins, Hairless (CG5460, CT36411, CT17282) is a transcriptional co-repressor that contains a "Paired box" domain, a KX-repeat domain, a NEDL-repeat domain and a serine-rich domain. Hairless is an antagonist of the Notch signaling pathway in Drosophila.

NCBI and Uniprot Product Information

• NCBI GI #: 22547204
• NCBI Accession #: NP_005135.2
• NCBI GenBank Nucleotide #: NP_005135.2
• UniProt Accession #: O43593
• Molecular Weight: ~ 127kDa
• NCBI Official Full Name: lysine-specific demethylase hairless isoform a
• UniProt Protein Name: Protein hairless
• UniProt Gene Name: HR
• UniProt Entry Name: HAIR_HUMAN

Uniprot Description

HR: May act as a transcription factor that could act on to regulate one of the phases of hair growth. Defects in HR are the cause of alopecia universalis congenita (ALUNC). ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair. Defects in HR are the cause of atrichia with papular lesions (APL); also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair. Defects in HR are the cause of hypotrichosis type 4 (HYPT4). An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nuclear body; nucleus

Molecular Function: DNA binding; metal ion binding; oxidoreductase activity; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent

Disease: Hypotrichosis 4; Alopecia Universalis Congenita; Atrichia With Papular Lesions