Rabbit anti-Human SCRIB Polyclonal Antibody | anti-SCRIB antibody

SCRIB Polyclonal Antibody

Cat. Num. AAA3006725

• Gene Names: SCRIB; CRIB1; SCRB1; SCRIB1; Vartul
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SCRIB; Polyclonal Antibody; SCRIB Polyclonal Antibody; Protein scribble homolog; Scribble; hScrib; Protein LAP4; CRIB1; KIAA0147; LAP4; SCRB1; VARTUL; anti-SCRIB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SCRIB polyclonal antibodydetects endogenous levels of SCRIB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1630

• Applicable Applications for anti-SCRIB antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human CELSR3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SCRIB polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug))

Related Product Information for anti-SCRIB antibody

Drosophila melanogaster genes, which are categorized based on the type of protein for which they encode, represent six major classifications, including intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) and other functional proteins. Morphogenesis and cell differentiation in Drosophila requires accurate control of cell division. Discs large (DLG), Scribble (Scrib) and Lethal giant larvae (LGL) tumor suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. DLG/Scrib/LGL proteins show apical cortical enrichment at prophase/metaphase and have a uniform cortical distribution. Mutations in the genes encoding multi-PDZ (PSD-95, discs-large and ZO-1) and the leucine-rich-repeat protein Scrib cause aberrant cell shapes and the loss of monolayer organization of embryonic epithelia. The human homolog, hScrib, is intracellularly localized to the vertebrate tight junction, which functions to correctly place adherens junctions. The PDZ domains of Scrib are predicted to bind to the consensus S/TXV at the C-terminus of proteins. PDZ domain proteins have been implicated at several different sites of the protein trafficking pathway, suggesting that Scrib is required for the localization of several epithelial determinants.

NCBI and Uniprot Product Information

• NCBI GI #: 355390315
• NCBI GeneID: 23513
• NCBI Accession #: NP_056171
• NCBI GenBank Nucleotide #: NP_056171.3
• UniProt Accession #: Q14160
• Molecular Weight: ~ 240kDa
• NCBI Official Full Name: protein scribble homolog isoform b
• NCBI Official Synonym Full Names: scribble planar cell polarity protein
• NCBI Official Symbol: SCRIB
• NCBI Official Synonym Symbols: CRIB1; SCRB1; SCRIB1; Vartul
• NCBI Protein Information: protein scribble homolog
• UniProt Protein Name: Protein scribble homolog
• Protein Family: Protein
• UniProt Gene Name: SCRIB
• UniProt Synonym Gene Names: CRIB1; KIAA0147; LAP4; SCRB1; VARTUL; Scribble; hScrib
• UniProt Entry Name: SCRIB_HUMAN

NCBI Description

This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]