Rabbit N-CoR Polyclonal Antibody | anti-N-CoR antibody

N-CoR (H76) Polyclonal Antibody

Cat. Num. AAA3001119

• Gene Names: NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: N-CoR; Polyclonal Antibody; N-CoR (H76) Polyclonal Antibody; Nuclear receptor corepressor 1; N-CoR1; NCOR1; KIAA1047; anti-N-CoR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NCoR1 (H76) polyclonal antibody detects endogenous levels of NCoR1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 914

• Applicable Applications for anti-N-CoR antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 51-100 of Human N-CoR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of N-CoR (H76) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of N-CoR (H76) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-N-CoR antibody

Retinoids are metabolites of vitamin A (retinol) and are believed to represent important signaling molecules during vertebrate development and tissue differentiation.Two families of retinoid receptors have been identified. Retinoic acid receptors (RARs), include RARalpha, RARbeta and RARgamma, each of which have a high affinity for all-trans retinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. Two cofactors that function to repress transcription, designated SMRT and N-CoR, have been shown to associate with the thyroid receptor and RAR in their unliganded state and are released from them upon ligand binding. The carboxy termini of both proteins contain receptor interacting domains while their amino termini contain two previously undescribed repressor domains. SMRT (silencing mediator for RARs and TRs) is 1495 amino acids in length with an estimated molecular weight of 168 kDa. N-CoR (nuclear receptor corepressor) is a protein 2453 amino acids in length with a relative molecular weight of 270 kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 298919179
• NCBI GeneID: 9611
• NCBI Accession #: NP_001177367.1
• NCBI GenBank Nucleotide #: NP_001177367.1
• UniProt Accession #: O75376
• Molecular Weight: ~ 270kDa
• NCBI Official Full Name: nuclear receptor corepressor 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor corepressor 1
• NCBI Official Symbol: NCOR1
• NCBI Official Synonym Symbols: N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
• NCBI Protein Information: nuclear receptor corepressor 1
• UniProt Protein Name: Nuclear receptor corepressor 1
• UniProt Gene Name: NCOR1
• UniProt Synonym Gene Names: KIAA1047; N-CoR; N-CoR1
• UniProt Entry Name: NCOR1_HUMAN

NCBI Description

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010]

Uniprot Description

N-CoR1: is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9. Increased expression correlates with the loss of vitamin D responsiveness in aggressive androgen-independent prostate cancer cells. Its gene tends to be overexpressed in multiple-myeloma cell lines.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; Sin3 complex; membrane; spindle microtubule; nuclear chromatin; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein binding; histone deacetylase regulator activity; sequence-specific DNA binding; histone deacetylase binding; thyroid hormone receptor binding; chromatin binding; transcription factor activity; transcription corepressor activity; nuclear hormone receptor binding

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; negative regulation of JNK cascade; CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; regulation of multicellular organism growth; chromatin modification; thalamus development; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; spindle assembly; positive regulation of histone deacetylation; cholesterol homeostasis; transforming growth factor beta receptor signaling pathway; gene expression; circadian regulation of gene expression; negative regulation of phosphoinositide 3-kinase cascade