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Tumor Necrosis Factor Alpha (TNFa) Recombinant Protein | TNFa recombinant protein
Recombinant Tumor Necrosis Factor Alpha (TNFa)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYLDLAESGQVYF GIIAL
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
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NCBI and Uniprot Product Information
Uniprot Description
Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells
By similarity.
Subunit structure: Homotrimer. Interacts with SPPL2B
By similarity.
Subcellular location: Cell membrane; Single-pass type II membrane protein
By similarity. Tumor necrosis factor, membrane form: Membrane; Single-pass type II membrane protein
By similarity. Tumor necrosis factor, soluble form: Secreted
By similarity. C-domain 1: Secreted
By similarity. C-domain 2: Secreted
By similarity.
Post-translational modification: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space
By similarity.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1
By similarity.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid
By similarity.
Sequence similarities: Belongs to the tumor necrosis factor family.
Research Articles on TNFa
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Product Notes
The TNFa tnf (Catalog #AAA2010817) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tumor Necrosis Factor Alpha (TNFa) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the TNFa tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Alpha (TNFa), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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