Testing Data
Rabbit anti-Human HAI-2 Polyclonal Antibody | anti-HAI-2 antibody
Anti-HAI-2 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-2, Placental Bikunin, SPINT2), Between KD1 and KD2 Antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
HAI-2; Polyclonal Antibody; Anti-HAI-2 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-2; Placental Bikunin; SPINT2); Between KD1 and KD2 Antibody; anti-HAI-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is specific for HGFA Inhibitor 2. It will not recognize HGFA Inhibitor 1
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
106
Applicable Applications for anti-HAI-2 antibody
Western Blot (WB)
Application Notes
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Detects a band of approximately 22-32 kDa due to post-translational modifications (predicted molecular weight: 28 kDa). Dilution optimised using Chromogenic detection.
Immunogen
Synthetic peptide based on the human HGFA Inhibitor 2 sequence.
Cellular Localization
Cell Membrane; Single-Pass Type I Membrane Protein and Secreted.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-HAI-2 antibody
Description: The antibody is a rabbit polyclonal antibody made to the region between Kunitz Domain-1 and Kunitz Domain-2 of human HAI-2. The antibody is made to a synthetic peptide based on the human sequence, and will recognize HAI-2 and not HAI-1. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. HAI-2 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HAI-2 also inhibits trypsin, and MTSP-1 (Matriptase-1), a serine proteinase that also activates HGF, and HGF-2 has been shown to inhibit hepsin with nanamolar kIs. HAI-2 was first known as bikunin, and SPINT2 (Serine Proteinase Inhibitor-2), a proteinase inhibitor found in amniotic fluid. HAI-2 message predicts a protein of 252 amino acids, with a predicted mass of 28.23 kDa and a pI of 9.54. A protein termed HAI-2 related small protein is a 106 amino acid protein with little homology to HAI-2, and the HAI-2 antibodies do not recognize the HAI-2 RSP. The HAI proteins are glycosylated, and may have other post-translational modifications, thus the apparent molecular weight is 22-32 Kda kDa for the full-length HAI-2. The chondroitin sulfate groups attached to HAI-2 are thought to be important for its function. The HAI-2 antibodies do not recognize HAI-1. Mice treated with HAI-2 in an ovarian cancer model had smaller tumors, and HAI-2 is reported to be elevated in macrophages during ovarian cancer, and this is considered a favorable indicator of survival. HAI-2 is secreted and membrane-inserted via a type-I transmembrane domain. The carboxyterminal end is a cytoplasmic domain, thought to interact with signaling and cytoskeletal machinery. The extracellular domains include two kringle domains (KD1 and KD2), and KD2 is thought to be inactive or with much lower activity against HGF. In mice the dominant HAI-2 lacks the first kringle domain, but the two-domain form dominates in humans, although in human testis a shorter HAI-2 has been reported, using a second transcription site. HAI-2 is shed from the cell surface by cleavage extracellular to the transmembrane domain. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Relevance: HGFA Inhibitor 2 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 2 also inhibits trypsin, and MTSP-1 (Matriptase-1), a serine proteinase that also activates HGF, and HGF-2 has been shown to inhibit hepsin with nanamolar kIs. HGFA Inhibitor 2 was first known as bikunin, and SPINT2 (Serine Proteinase Inhibitor-2), a proteinase inhibitor found in amniotic fluid.
Relevance: HGFA Inhibitor 2 is an endogenous inhibitor of the kunitz-type serine proteinase HGF-activator (HGFA). HGF was first described as a hepatocyte-specific mitogen and survival factor, and has since been shown to exert a variety of actions on many cell types by binding to its MET receptor. HGF is activated by cleavage of the single-chain form to form a two-chain version by HGFA. HGFA Inhibitor 2 also inhibits trypsin, and MTSP-1 (Matriptase-1), a serine proteinase that also activates HGF, and HGF-2 has been shown to inhibit hepsin with nanamolar kIs. HGFA Inhibitor 2 was first known as bikunin, and SPINT2 (Serine Proteinase Inhibitor-2), a proteinase inhibitor found in amniotic fluid.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HAI-2 related small protein
Similar Products
Product Notes
The HAI-2 (Catalog #AAA4157561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HAI-2 (HGFA (Hepatocyte Growth Factor Activator) Inhibitor-2, Placental Bikunin, SPINT2), Between KD1 and KD2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAI-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Detects a band of approximately 22-32 kDa due to post-translational modifications (predicted molecular weight: 28 kDa). Dilution optimised using Chromogenic detection. Researchers should empirically determine the suitability of the HAI-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAI-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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