Human CUBN (Cubilin) ELISA Kit | CUBN elisa kit

Human CUBN (Cubilin) ELISA Kit

Cat. Num. AAA7608162

• Gene Names: CUBN; IFCR; MGA1; gp280
• Reactivity: Human
• Synonyms: CUBN (Cubilin); Human CUBN (Cubilin) ELISA Kit; Cubn; gp280; IFCR; MGA1; cubilin; intrinsic factor-cobalamin receptor; CUBN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CUBN. No significant cross-reactivity or interference between CUBN and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CUBN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 126091152
• NCBI GeneID: 8029
• NCBI Accession #: NP_001072.2
• NCBI GenBank Nucleotide #: NM_001081.3
• UniProt Accession #: O60494; Q5VTA6; Q96RU9; B0YIZ4
• Molecular Weight: 398736 MW
• NCBI Official Full Name: cubilin
• NCBI Official Synonym Full Names: cubilin
• NCBI Official Symbol: CUBN
• NCBI Official Synonym Symbols: IFCR; MGA1; gp280
• NCBI Protein Information: cubilin
• UniProt Protein Name: Cubilin
• Protein Family: Cubilin
• UniProt Gene Name: CUBN
• UniProt Synonym Gene Names: IFCR

NCBI Description

Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.