Rabbit Matriptase-2 Polyclonal Antibody | anti-TMPRSS6 antibody

Anti-Matriptase-2 (MTSP-2, TMSP6, PVAE354) Catalytic Domain Antibody

Cat. Num. AAA4157573

• Gene Names: TMPRSS6; IRIDA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Matriptase-2; Polyclonal Antibody; Anti-Matriptase-2 (MTSP-2; TMSP6; PVAE354) Catalytic Domain Antibody; anti-TMPRSS6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Reacts with Matriptase 2, and does not recognize matriptase 1.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 802

• Applicable Applications for anti-TMPRSS6 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000. 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Detects a band of approximately 95 kDa (secreted Matriptase 2) under reducing conditions (predicted molecular weight: 89 kDa). Dilution optimised using Chromogenic detection.
• Immunogen: Synthetic peptide based on the catalytic domain of Human Matriptase 2.
• Cellular Localization: Cell Membrane (Single Pass Type II Membrane Protein) and Secreted.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-TMPRSS6 antibody

Description: The antibody is a polyclonal antibody made to the Type-II transmembrane serine protease matriptase-2. The antibody is made to a synthetic peptide based on the catalytic domain of human matriptase-2. The antibody reacts with human, mouse and rat matriptase-2, and does not recognize matriptase-1. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. Matriptase-2, also known as MTSP-2 (membrane-type serine proteinase-2), TMSP6 (Type-II Membrane Serine Proteinase-6) and PVAE354, is a type-II serine proteinase. The full-length human matriptase-2 is an 802 amino acid protein with predicted (unmodified) mass of 88.9 kDa, and a pI of 6.04. Secreted Matriptase-2 is glycosylated, and runs at approximately 95 kDa on reduced SDS-PAGE gels. Matriptase-2 shares approximately 37% identity with matriptase-1 and matriptase-3, mostly in the catalytic domain. Matriptase-2 is an S1A family proteinase, in the PA clan of serine proteinases, which includes trypsin, chymotrypsin, acrosin, and many other key enzymes. A literature alias exists for MTSP-2, the Mouse Serine-Type Proteinase, granzyme-A. Matriptase-2 is organized by an aminoterminal cytoplasmic domain, followed by a transmembrane domain, a stem region and then two CUB domains, three LDL receptor-like motifs, and a carboxyterminal catalytic domain. Like matriptase-1, matriptase-2 is shed from the membrane by a cleavage in the stem region, and the catalytic domain can be cleaved from the regulatory domains to generate a free trypsin-like enzyme. The liberated catalytic domain is also detected by the RP3-Matriptase-2 antibody. Matriptase-2 may be more limited in distribution than matriptase-1, and has been identified in the liver by message, but appears to be in kidney, liver and uterus, and in a number of cell lines. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Relevance: Matriptase 2 is a type II serine proteinase. Matriptase 2 is an S1A family proteinase, in the PA clan of serine proteinases, which includes trypsin, chymotrypsin, acrosin, and many other key enzymes. A literature alias exists for MTSP2, the Mouse Serine Type Proteinase, granzyme A. Matriptase 2 is organized by an aminoterminal cytoplasmic domain, followed by a transmembrane domain, a stem region and then two CUB domains, three LDL receptor like motifs, and a carboxyterminal catalytic domain. Like matriptase 1, matriptase 2 is shed from the membrane by a cleavage in the stem region, and the catalytic domain can be cleaved from the regulatory domains to generate a free trypsin like enzyme. Matriptase 2 may be more limited in distribution than matriptase 1, and has been identified in the liver by message, but appears to be in kidney, liver and uterus, and in a number of cell lines. The free catalytic domain is also detected by this antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 23477115
• NCBI GeneID: 164656
• NCBI Official Full Name: matriptase-2
• NCBI Official Synonym Full Names: transmembrane serine protease 6
• NCBI Official Symbol: TMPRSS6
• NCBI Official Synonym Symbols: IRIDA
• NCBI Protein Information: transmembrane protease serine 6
• Protein Family: Transmembrane protease serine

NCBI Description

The protein encoded by this gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]